Hypertrophy, Physical Performance
Conditions
Brief summary
Protein ingestion timing is an important component for muscle accretion. The investigators hypothesized that protein ingestion timing could affect muscular adaptations and performance in resistance-trained men.
Interventions
DIETARY_SUPPLEMENTisolate whey protein prior to and immediately after resistance training
This arm involved protein ingestion immediately prior to and immediately after resistance training for 12 weeks. Participants will consume 40 g of isolate whey protein at their recommended timing.
DIETARY_SUPPLEMENTisolate whey protein 3 hours prior to and 3 hours after resistance training
This arm involved protein ingestion 3 hours prior to and 3 hours after resistance training for 12 weeks. Participants will consume 40 g of isolate whey protein at their recommended timing.
Sponsors
Shahid Beheshti University of Medical Sciences
Marymount University
Study design
Allocation
RANDOMIZED
Intervention model
PARALLEL
Primary purpose
BASIC_SCIENCE
Masking
TRIPLE (Subject, Investigator, Outcomes Assessor)
Eligibility
Sex/Gender
MALE
Age
18 Years to 35 Years
Healthy volunteers
Yes
Inclusion criteria
* performing resistance training at least three times a week for 1 year prior to the start of the study * not taking any steroids or any illegal agents known to increase muscle size for at least 1 year prior to the start of the study * no known medical issues * sleeping for at least seven to eight hours during the 24-hour day
Exclusion criteria
* non-willingness to continue protein ingestion or exercise protocols * participation in exercise other than the prescribed resistance training program * consumption of dietary supplement (other than the protein intervention) during the study period * Missing more than one resistance training session or protein ingestion throughout the study period
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Body Composition | 12 weeks | Total Lean mass, fat mass, and estimated VAT (g) will be assessed using whole-body DXA |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| Muscular Endurance | 12 weeks | Using leg- and chest press exercises at 75% of the 1-RM |
| Jump testing | 12 weeks | Using the vertical and broad jump tests |
| Pull-ups | 12 weeks | Using the pull-up test |
| Muscular Strength | 12 weeks | Using 1 repetition maximum |
| Creatinine and Urea Nitrogen | 12 weeks | Urine samples will be collected using standard procedures |
| Cholesterol Levels | 12 weeks | Using Fasting blood samples |
| Liver enzymes (ALT, AST, and GGT) | 12 weeks | Using Fasting blood samples |
Countries
United States
Outcome results
None listed