Sepsis, Severe
Conditions
Brief summary
The etiological diagnosis of sepsis is the key to guide clinical treatment. Metagenomic sequencing (mNGS) is very suitable for the diagnosis of sepsis due to its rapid, accurate and not easy to be disturbed by the environment. However, the conventional pathogen mNGS has potential risks such as low detection rate, loss of intracellular bacteria and fungi. At present, the latest fully targeted pathogen capture mNGS technology makes up for the shortcomings of conventional methods by bidirectional enrichment of pathogen nucleic acids. The aim of this study was to explore the value of fully targeted pathogen capture mNGS in improving etiological diagnosis in patients with sepsis compared with conventional methods.
Detailed description
This study planned to continuously collect samples from patients with sepsis who were admitted to the intensive care Unit of our hospital from 2022.08 to 2024.07, and conduct whole-target pathogen capture mNGS detection and follow-up etiological diagnosis based on blind method. The clinical diagnosis with clear etiology was taken as the gold standard.
Interventions
None listed
Sponsors
Shenzhen Second People's Hospital
Study design
Observational model
OTHER
Time perspective
CROSS_SECTIONAL
Eligibility
Sex/Gender
ALL
Age
18 Years to No maximum
Healthy volunteers
No
Inclusion criteria
* Critical care patients aged ≥ 18 years old * Patients with sepsis
Exclusion criteria
* Lack of traditional pathogen detection results * Discharge diagnosis without clear etiology
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| sensitivity | up to 3 year | The chance of testing positive for a pathogen in a sample judged by the gold standard |
| specificity | up to 3 year | The chance of a negative test in a sample judged pathogen-free by the gold standard |
Contacts
Primary ContactNi Xie
Outcome results
None listed