Ageing
Conditions
Brief summary
The main goal of this study is to determine the difference in amino acid digestibility of milk, sorghum and black beans between older (65-80 years) and younger (20-35 years) adults using the dual tracer method.
Detailed description
Older adults should meet their amino acid requirements to prevent development of sarcopenia. Dietary protein quality is of importance, as determined by amino acid composition and digestibility of the consumed protein. There is a need to investigate the impact of ageing on amino acid digestibility and thus quality in vivo. The dual tracer method is an indirect, minimal-invasive method to determine amino acid digestibility in humans. It is expected that amino acid digestibility decreases with age and that the effect between age groups is greater for poorly digestible protein sources. In this randomized, cross-over study, the researchers will determine the difference in amino acid digestibility of milk, sorghum and black beans between older (65-80 years) and younger (20-35 years) adults using the dual tracer method. Secondary objectives concern the difference in amino acid kinetics between older and younger adults and the association between gut health plasma markers and amino acid digestibility.
Interventions
OTHERMilk
Twenty grams of the deuterium (2H) - labelled skimmed milk powder mixed with water will be consumed on one of the three different test days. The meal will be divided in 10 portions, consumed every hour. The 2H-labelled protein source is mixed with free 13C-labelled amino acids as reference. Protein free cookies are served alongside to equal macronutrient composition with the other two protein sources.
OTHERSorghum
Twenty grams of the deuterium (2H) - labelled whole grain sorghum cooked with water will be consumed on one of the three different test days. The meal will be divided in 10 portions, consumed every hour. The 2H-labelled protein source is mixed with free 13C-labelled amino acids as reference.
OTHERBlack beans
Twenty grams of the deuterium (2H) - labelled black beans cooked with water and mashed into a puree, will be consumed on one of the three different test days. The meal will be divided in 10 portions, consumed every hour. The 2H-labelled protein source is mixed with free 13C-labelled amino acids as reference. A carbohydrate drink is served alongside to equal macronutrient composition with the other two protein sources.
Sponsors
Wageningen University
Study design
Allocation
RANDOMIZED
Intervention model
CROSSOVER
Primary purpose
PREVENTION
Masking
SINGLE (Investigator)
Intervention model description
Participants will have three test days, each separated by two weeks. The participants will randomly consume one of the three protein sources on each test day respectively.
Eligibility
Sex/Gender
ALL
Age
20 Years to 80 Years
Healthy volunteers
Yes
Inclusion criteria
* Age: 20-35 years or 65-80 years * Body Mass Index (BMI): 20.0 - 30.0 kg/m2 * Veins suitable for blood sampling * Healthy as assessed with a questionnaire * Regular and normal Dutch eating habits as assessed with a questionnaire * Having given written informed consent * Willing to comply with study procedures * Accept use of all encoded data, including publication, and the confidential use and storage of all data for 15 years.
Exclusion criteria
* Chronic disease, for example: * Diabetes mellitus, being treated for high blood glucose or increased fasting blood glucose (\> 6.7 mmol/l in finger prick blood) * Active cardiovascular disease * Hepatic disease (e.g. hepatitis) * Renal disease * Cancer * Bowel disease (e.g. inflammatory bowel disease, ulcers, bleeding) * Pancreatitis * History of medical or surgical events that may affect GI function and the study outcomes, for example: * Bariatric surgery * Gastrointestinal tract surgery * Digestive tract disorder * Chewing problems * Medicine use that interferes with GI function and the study outcomes, for example: * Glucose lowering drugs * Proton pump inhibitors * Laxatives * Habits that interfere with the study outcomes: * Probiotics and/or protein supplement use * Smoking * Drug abuse * Alcohol consumption for men \>21 units/week and \>4/day and for women \>14 units/week and \>3/day * Following a weight-loss diet, medically prescribed diet or other diet with a low calorie intake or an unbalanced nutrient intake like a vegan or very low carbohydrate diet * Moderate to high intense physical activity for more than 5 hours a week * Difficulties with eating breakfast in the morning * Other: * Self-reported allergy or intolerance to the tested products (milk, sorghum, black beans) * Weight loss of more than 3 kg in the month prior to study screening * Being pregnant * For men: Hb \<8,5 mmol/l and for women: Hb \<7,5 mmol/l * Recent blood donation (\<2 months prior to start of the study) * Current participation in other research and \<2 months previous participation in other research * Not having a general practitioner * Not willing to accept information-transfer concerning participation in the study, or information regarding his or her health, like laboratory results and eventual adverse events to and from his general practitioner * Working at the department of Human Nutrition and Health at Wageningen University & Research
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Amino acid digestibility of three different protein sources | 1 measurement repeated on 3 test days for 3 protein sources respectively, separated by 2 weeks wash-out | Ratio between meal and plasma isotope enrichment in amino acids |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| Linear increase in plasma amino acids of three different protein sources | 11 blood samples during 8 hours, repeated on 3 test days for 3 protein sources respectively, separated by 2 weeks wash-out | Amino acids in plasma are determined over time. First measure of kinetics is the linear increase or the slope of the curve. |
| Area under the curve for plasma amino acid concentration of three different protein sources | 11 blood samples during 8 hours, repeated on 3 test days for 3 protein sources respectively, separated by 2 weeks wash-out | Amino acids in plasma are determined over time. Second measure of kinetics is the area under the curve. |
| Steady state plasma amino acid concentration of three different protein sources | 11 blood samples during 8 hours, repeated on 3 test days for 3 protein sources respectively, separated by 2 weeks wash-out | Amino acids in plasma are determined over time. Third measure of kinetics is the steady state concentration or the plateau of the curve. |
| Plasma gut health markers of the participants in relation to age and amino acid digestibility | 1 blood sample before consumption of the protein source, repeated on 3 test days for 3 protein sources respectively, separated by 2 weeks wash-out | Four gut health markers in plasma will be measured: kynurenine/tryptophan ratio, citrulline, ornithine, I-FABP. |
Countries
Netherlands
Outcome results
None listed