Alzheimer Disease, Lewy Body Disease, Neurodegenerative Diseases
Conditions
Brief summary
The subject uses cytof to analyze PBMC of sporadic AD and DLB, which is used to reveal the differences in immune characteristics of the two diseases at the single-cell level, build immune models for specific diseases, and define these two neurodegenerative diseases with high precision from the level of molecular immunity. To provide basis for further study of the immunohistochemical differences between the two diseases, and provide objective support for clinical diagnosis and differential diagnosis.
Interventions
BIOLOGICALDetection of cytof in peripheral PBMC
Each subject needs to take 5ml of peripheral venous blood to extract PBMC cells, and then conduct cytof detection. This is a mass spectrometry flow cytometry method based on single cell level. It has the ability to analyze all immune cells with high resolution.
DRUGDonepezil for patients with AD and DLB
The subjects in AD and DLB groups were given donepezil at an initial dose of 5mg and then increased to 10mg one month later. Lasting for 1 year.
Sponsors
First Affiliated Hospital of Zhejiang University
Study design
Allocation
NON_RANDOMIZED
Intervention model
PARALLEL
Primary purpose
DIAGNOSTIC
Masking
NONE
Eligibility
Sex/Gender
ALL
Age
55 Years to 100 Years
Healthy volunteers
Yes
Inclusion criteria
1. AD inclusion criteria: a.Clinical diagnosis of Alzheimer's Disease 2. DLB inclusion criteria: 1. Clinical diagnosis of Dementia with Lewy bodies
Exclusion criteria
1. Infectious diseases 2. autoimmune disease 3. heart failure 4. chronic obstructive pulmonary disease (COPD) 5. cancer 6. renal failure 7. recently major surgery 8. alcohol and / or drug abuse 9. disturbance of consciousness 10. clinical diagnosis of major depression disorder 11. clinical diagnosis of Anxiety disorder
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Immunerepertorie | 1 year | The immune cells were drawn into immunoreceptor by mass cytometry (cytof) technology. Immunerepertorie is used for the classification of immune cells. According to the proportion of various immune cell subtypes and biomarkers in all cells, it is used to find the difference between immune cell subtypes and biomarkers in different diseases. Scoring frame: 0-1. The higher the score, the greater the proportion of this subtype or biomarker. |
| immunological Elastic-Net (iEN) model | 1 year | The immunological Elastic-Net (iEN), which incorporates immunological knowledge directly into the predictive models. The ROC curve output by the model is plotted with specificity as the abscissa and sensitivity as the ordinate, and the AUC (area under the curve) value is calculated. The AUC value was used to evaluate the authenticity of the IEN model. Score frame: 0.5-1. A higher value indicates that the model is closer to the real situation. |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| Mini-mental State Examination（MMSE） | 1 year | MMSE was used to evaluate the degree of intellectual status and cognitive impairment. Score frame: 0 \ 30. The lower the score, the more severe the cognitive impairment. |
Countries
China
Contacts
Primary ContactHu ShaoHua
Backup ContactQiu CongLong
Outcome results
None listed