Lifestyle, Sedentary
Conditions
Brief summary
The purpose of this study is to compare the impact of high-intensity interval training (HIIT) versus moderate-intensity continuous training (MICT) on human health outcomes in healthy sedentary subjects, over 12 weeks of exercise training. The investigators will compare several health parameters, such as changes in multiomics profile, cardiorespiratory fitness, muscle strength, and body composition, before and after 12-week interventions of either HIIT or MICT.
Interventions
OTHERExercise intervention
12 weeks of exercise training, 3 times per week, \ 1h long
Sponsors
Stanford University
Study design
Allocation
RANDOMIZED
Intervention model
PARALLEL
Primary purpose
OTHER
Masking
NONE
Eligibility
Sex/Gender
ALL
Age
18 Years to 65 Years
Healthy volunteers
Yes
Inclusion criteria
* Be 18-65 years of age * Not be pregnant or lactating in the last 12 months, or planning to become pregnant for the next 4 months. A pregnancy test will be performed on the day of DXA scan in women of child-bearing potential. Not be post-partum during the last 12 months. * Be generally healthy (a list of medical conditions and/or medication that will exclude participants is listed in the section below) * Body mass index (BMI) \> 18 to \< 40 kg/m2 * Sedentary in the past year, defined as regular (structured) endurance exercise (e.g., brisk walking, jogging, running, cycling, elliptical, or swimming activity that results in feelings of increased heart rate, rapid breathing, and/or sweating) or resistance training (resulting in muscular fatigue), no more than 1 hour per week. Persons bicycling as a mode of transportation to and from work \>1 day/week etc. will not be considered sedentary. Leisure walkers are included unless they meet the heart rate, breathing, and sweating criteria noted above.
Exclusion criteria
*
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Cardiopulmonary Exercise Testing | Baseline; 12 weeks | Change in VO2 max after training, measured during cardiopulmonary exercise testing |
| Clinical markers | Baseline; 12 weeks | Changes in measured lipids in mg/dL including total cholesterol, LDL cholesterol, HDL cholesterol, and triglycerides |
| Transcriptome after 12-week intervention | Baseline; 12 weeks | Change in mRNA-based expression measured in biospecimens after 12 weeks of training |
| Proteome after 12-week intervention | Baseline; 12 weeks | Change in protein measured in biospecimens after 12 weeks of training |
| Metabolome after 12-week intervention | Baseline; 12 weeks | Change in metabolites measured in biospecimens after 12 weeks of training |
| Lipidome after 12-week intervention | Baseline; 12 weeks | Change in lipids measured in biospecimens after 12 weeks of training |
| Epigenome after 12-week intervention | Baseline; 12 weeks | Change in epigenome measured in biospecimens after 12 weeks of training |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| Leg strength | Baseline; 12 weeks | Change in leg strength measured by Biodex |
| Hand grip strength | Baseline; 12 weeks | Change in hand grip strength measured by Biodex |
| Resting heart rate | Baseline; 12 weeks | Change in resting heart rate measured by wearable devices |
| Metagenome profile | Baseline; 12 weeks | Change in metagenome profile from stool microbiome kit |
| Blood glucose | Baseline; 12 weeks | Change in blood glucose in mg/dl profile measured with continuous glucose monitor |
| Left ventricle (LV) mass | Baseline; 12 weeks | Change in left ventricle mass measured by echocardiogram |
| Metabolome after acute exercise | Baseline; 12 weeks | Change in metabolites measured in biospecimens after acute exercise tests |
| Ejection fraction | Baseline; 12 weeks | Change in ejection fraction measured by echocardiogram |
| Exercise Motivation | Baseline; 12 weeks | Change in exercise motivation level measured by motivation for physical activity (RM 4-FM) questionnaire. RM 4-FM scores four different dimensions of motivation, each on a 0-7 point composite scale, with higher scores indicating stronger motivation. |
| Exercise Self-Efficacy | Baseline; 12 weeks | Change in exercise self-efficacy measured by Exercise Self Efficacy questionnaire, , a 9-item scale providing a 9-28 point composite score, with higher scores indicating higher self-efficacy for exercise. |
| Depression | Baseline; Week 4, 8, 12 | Change in depression presence and/or severity measured by Patient Health Questionnaire-8, an 8-item scale providing a 0-24 point composite score, with higher scores indicating greater severity. |
| Generalized Anxiety | Baseline; Week 4, 8, 12 | Change in anxiety presence and/or severity measured by Generalized Anxiety Disorder-7 , a 7-item scale providing a 0-21 point composite score, with higher scores indicating greater anxiety. |
| Perceived Stress | Baseline; Week 4, 8, 12 | Change in perceived stress measured by Perceived Stress Scale-10, a 10-item scale providing a 0-40 point composite score, with higher scores indicating greater perceived stress. |
| LV diastolic volume | Baseline; 12 weeks | Change in LV diastolic volume measured by echocardiogram |
| Transcriptome after acute exercise | Baseline; 12 weeks | Change in mRNA-based expression measured in biospecimens after acute exercise tests |
| Proteome after acute exercise | Baseline; 12 weeks | Change in protein measured in biospecimens after acute exercise tests |
| Lipidome after acute exercise | Baseline; 12 weeks | Change in lipids measured in biospecimens after acute exercise tests |
| Epigenome after acute exercise | Baseline; 12 weeks | Change in epigenome measured in biospecimens after acute exercise tests |
| Lean muscle mass | Baseline; 12 weeks | Change in lean muscle mass in kg measured by dual-energy x-ray absorptiometry |
| Fat mass | Baseline; 12 weeks | Change in fat mass in kg measured by dual-energy x-ray absorptiometry |
Countries
United States
Outcome results
None listed