Recurrent Pregnancy Loss, Not Pregnant, Vaginal Microbiome
Conditions
Keywords
Microbiome, Recurrent Pregnancy Loss
Brief summary
The female genital tract microbiome may reflect female reproductive health and may be related to pregnancy outcomes. Disturbances in this microbiome may be associated with adverse reproductive outcomes. The investigators hypothesize that the endometrial and vaginal microbiome composition in women with a history of recurrent pregnancy loss are different, compared with those in normal fertile women.
Detailed description
The investigators want to describe the endometrial microbiome composition in normal fertile women and women with a history of recurrent pregnancy loss by analyzing mid-luteal phase vaginal swab and endometrial biopsy samples using 16S rRNA sequencing.
Interventions
OTHERVaginal swab and endometrial biopsy
A vaginal swab (DNA/RNA shield collection tube with swab - Zymo Research, CatNo R1107) and endometrial biopsy (Endobrush® - Lab CCD, Paris, France) will be obtained during 18th - 22nd day of the menstrual cycle.
Sponsors
Mỹ Đức Hospital
Study design
Observational model
COHORT
Time perspective
PROSPECTIVE
Eligibility
Sex/Gender
FEMALE
Age
18 Years to 38 Years
Healthy volunteers
Yes
Inclusion criteria
1. Recurrent pregnancy loss patients Inclusion Criteria: * 18-38 years old * Having a regular menstrual cycle, from 25 to 35 days * Having ≥ 2 recurrent pregnancy loss and * Couples with normal karyotype results * Having no condition causing pregnancy loss such as antiphospholipid syndrome, abnormal thyroid function. * Agree to participate in the study
Exclusion criteria
* Irregular menstrual cycle * Having uterine abnormalities (e.g., adenomyosis, intrauterine adhesions, unicornuate/ bicornuate/ arcuate uterus; unremoved hydrosalpinx, endometrial polyp, submucosal fibroid) * Using intrauterine device within the last 3 months * Using antibiotic/vaginal pessary/contraceptive pills within 2 weeks * Having sexual intercourse within 48 hours 2. Healthy control patients Inclusion Criteria: * 18-38 years old * Having a regular menstrual cycle, from 25 to 35 days * No history of pregnancy loss * Having 1 or more live birth, with the youngest child ≥ 6 months old * Agree to participate in the study
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Midluteal bacterial composition of endometrium in women with recurrent pregnancy loss and control group | 1 week | Bacterial composition in the endometrium in midluteal phase are analysed using 16S rRNA gene sequencing |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| Midluteal bacterial composition of vagina in women with recurrent pregnancy loss and control group | 1 week | Bacterial composition in the vaginal in midluteal phase are analysed using 16S rRNA gene sequencing |
| Phylogenetic tree of endometrial microorganisms in women with recurrent pregnancy loss and control group | 1 week | Phylogenetic tree based on 16S rRNA gene sequences |
| Phylogenetic tree of vaginal microorganisms in women with recurrent pregnancy loss and control group | 1 week | Phylogenetic tree based on 16S rRNA gene sequences |
Countries
Vietnam
Contacts
Primary ContactNam T Nguyen, MD
Backup ContactTuong M Ho, MD, MCE
Outcome results
None listed