Organ Protection
Conditions
Keywords
lidocaine, postoperative cognitive dysfunction, miRNA-135a, Rock2, Add1
Brief summary
The purpose of this study is to investigate the effects of intravenous lidocaine on serum miRNA-135a and its downstream proteins Rock2 and Add1 in elderly patients undergoing non-cardiac under general anesthesia.
Detailed description
Postoperative cognitive dysfunction (POCD) is a major complication following surgeries and anesthesia, especially in elderly individuals. Lidocaine, an inexpensive, widely available, and relatively safe compound, is a local anesthetic that readily crosses the blood-brain barrier. Intravenous lidocaine can reduce the incidence of POCD. However, the mechanism is still unclear. Rock2 and Add1, which are regulated by miRNA-135a, play a key role in learning, memory, and cognition. The objective of this study is to investigate the efficacy of intravenous lidocaine on the incidence of early POCD, and the levels of Rock2, Add1, and miRNA-135a in elderly patients undergoing non-cardiac under general anesthesia.
Interventions
DRUGLidocaine
Intravenous bolus of 1.5 mg/kg of lidocaine followed by a continuous infusion of 3.0 mg/kg for the frst hour, 1.5 mg/kg for the second hour, 0.7 mg/kg until the end of the surgery.
DRUGNormal saline
Patients are received equal volumes of saline intravenously until the end of the surgery
Sponsors
General Hospital of Ningxia Medical University
Study design
Allocation
RANDOMIZED
Intervention model
PARALLEL
Primary purpose
PREVENTION
Masking
DOUBLE (Subject, Caregiver)
Eligibility
Sex/Gender
ALL
Age
65 Years to 80 Years
Healthy volunteers
No
Inclusion criteria
* Patients undergoing elective non-cardiac surgery under general anesthesia * ASA physical status II-III * Aged ≥65 years
Exclusion criteria
* Serious heart, lung, liver and kidney diseases * Central nervous system injury * Mental illness and drug dependence * On regular use of analgesic/sedative/antidepressant * Unable to cooperate * Allergy to lidocaine * MMSE \< 23 points
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Changes from Baseline miRNA-135A at the end of surgery immediately and 1, 3days after surgery | at preoperation, at the end of surgery immediately, 1day and 3day after surgery | Venous blood was extracted, and the serum miRNA-135a level of patients were detected by PCR and ELISA before surgery, at the end of surgery, 1day and 3day after surgery |
| Changes from Baseline Rock2 at the end of surgery immediately and 1, 3days after surgery | at preoperation, at the end of surgery immediately, 1day and 3day after surgery | Venous blood was extracted, and the serum Rock2 level of patients were detected by PCR and ELISA before surgery, at the end of surgery, 1day and 3day after surgery |
| Changes from Baseline Add1 at the end of surgery immediately and 1, 3days after surgery | at preoperation, at the end of surgery immediately, 1day and 3day after surgery | Venous blood was extracted, and the serum Add1 level of patients were detected by PCR and ELISA before surgery, at the end of surgery, 1day and 3day after surgery |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| Changes from Baseline mini-mental state examination (MMSE) at the end of surgery immediately and 1, 3days after surgery | at preoperation,1day and 3day after surgery | MMSE was performed |
Other
| Measure | Time frame | Description |
|---|---|---|
| Postoperative cognitive dysfunction (POCD) | at preoperation,1day and 3day after surgery | the incidence of POCD |
Contacts
Primary ContactHanxiang Ma
Backup ContactLi Zhao
Outcome results
None listed