Infertility
Conditions
Keywords
Blastocyst diameter, PGT-A, Time lapse, Blastocyst survival, TE biopsy
Brief summary
Morphometric measurement of blastocyst diameter was taken on the day of trophoectoderm biopsy. PGT-A was done using NGS platform, followed by thawing and transfer of euploid blastocyts. Follow up of the Pregnancy and implantation rates is done and recorded to study the correlation between the diameter and reproductive outcomes of the blastocyst.
Detailed description
Blastocyst morphological grading proposed by Gardner and Schoolcraft including the expansion degree, inner cell mass, and trophoectoderm assessment has proved its importance. Blastocyst expansion degree that can presented in diameter is linked to the blastocyst implantation and overall success. In order to evaluate the importance of blastocyst diameter in PGT-A cases, measurements will be taken on the day of trophoectoderm biopsy TE (diameter in μm), followed by TE biopsy using flicking technique. Next, PGT-A will be done using NGS platform and results will be confirmed using PGT-ai. Thawing of euploid blastocyst will take place and expansion degree, survival rate will be recorded. Patient will be followed up for the clinical pregnancy and implantation rates.
Interventions
None listed
Sponsors
Ganin Fertility Center
Study design
Observational model
COHORT
Time perspective
PROSPECTIVE
Eligibility
Sex/Gender
ALL
Age
18 Years to 45 Years
Healthy volunteers
No
Inclusion criteria
* Female aged 18-45 years old * Male aged 18-60 years old * ICSI cases having PGT-A for their blastocysts * Must have their blastocysts cultured in time lapse incubator
Exclusion criteria
* Presence of any of the endometrial factors that affect embryo implantation such as hydrosalpings, adenomyosis or previous uterine infection * Any contradictions to undergoing in vitro fertilization or gonadotropin stimulation * Use of donor oocytes or sperm * Use of gestational carrier
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Blastocoele re-expansion degree | Two hours post thawing | Defined as the degree of blastocoele re-expansion and presented in % |
| Pregnancy rate | 15 days post embryo transfer | Defined as clinical pregnancy per transfer |
| Implantation rate | 6-8 weeks of pregnancy | Defined as number of gestational sacs with fetal heart beat, shown by ultrasound in gestational week 6 over number of embryo transferred. |
| Blastocyst survival rate | Two hours post thawing | Defined as the proportion of blastocysts that showed expansion degree ≥50% and their cells remained intact after thawing and re-expansion |
Countries
Egypt
Contacts
Primary ContactEman Hasanen, BSc.
Backup ContactManar Hozayen, MSc.
Outcome results
None listed