Asthma
Conditions
Brief summary
Asthma is one of the most common chronic respiratory diseases. Previous study found a series of differentially expressed genes and proteins (transgelin-2, metallothionein-2, ezrin) in asthmatic rat. The aim of the study is to investigate the changes and associations of these gene with asthma in human.
Detailed description
This study will investigate the changes and associations in asthma. In this project, the investigators will explore the changes of gene expression of transgelin-2, metallothionein-2 and ezrin in asthma and investigate their correlation with current diagnosis markers of asthma, including pulmonary function, fractional exhaled nitric oxide,eosinophil counts,immunoglobulin E, interleukin-5, etc.
Interventions
DIAGNOSTIC_TESTNo intervention
No intervention
Sponsors
Xiangya Hospital of Central South University
National Natural Science Foundation of China
Shanghai University of Traditional Chinese Medicine
Study design
Observational model
CASE_CONTROL
Time perspective
RETROSPECTIVE
Eligibility
Sex/Gender
ALL
Age
14 Years to 75 Years
Healthy volunteers
Yes
Inclusion criteria
1. Males or females, aged 14-75 years; 2. Patients with a definite diagnosis of bronchial asthma; 3. The diagnosis of asthma according to the Global Initiative for Asthma (GINA), with positive bronchodilator responsiveness (reversibility) test; 4. Healthy volunteers without respiratory symptoms such as cough, wheezing, chest tightness or dyspnea; 5. Agree with all procedures in this trial by signing a written informed consent form.
Exclusion criteria
1. Patients with chronic obstructive pulmonary disease or other airway disease; 2. Complicated with other severe primary diseases (including hypertension, cancer, hyperthyroidism, bronchiectasia, cardiac insufficiency) and conditions that would prevent participation in the trial or put the participant at risk; 3. Women who are known to be pregnant or breastfeeding; 4. Not willing to participate; 5. Psychiatric history and neurological disorders can not work normally; 6. Other conditions that the researcher considers inappropriate to participate in this study.
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Difference in gene expression of transgelin-2, metallothionein-2 and ezrin between controls and asthmatic patients | Two year | PCR test of gene expression of transgelin-2, metallothionein-2 and ezrin between controls and asthmatic patients |
| Association of transgelin-2, metallothionein-2 and ezrin with asthma | Two year | Correlation analysis between gene expressions of transgelin-2, metallothionein-2, ezrin and current asthmatic tests (pulmonary function, fractional exhaled nitric oxide,immunoglobulin E, serum eosinophil counts, interleukin-5, etc) |
Countries
China
Contacts
Primary ContactLei-Miao Yin, MD
Backup ContactJun-Tao Feng, MD
Outcome results
None listed