Muscle Atrophy
Conditions
Brief summary
The objectives of this study are to: 1. Establish whether combined β-lactoglobulin supplementation and resistance training for 1 week prior to 5 days of limb immobilisation will attenuate the decrease in integrated free-living rates of MPS during short-term muscle disuse. 2. Establish whether combined β-lactoglobulin supplementation and resistance training for 1 week prior to limb immobilisation will attenuate the decrease in muscle mass and strength during short-term muscle disuse.
Detailed description
The objectives of this study are to: 1. Establish whether combined β-lactoglobulin supplementation and resistance training for 1 week prior to 5 days of limb immobilisation will attenuate the decrease in integrated free-living rates of MPS during short-term muscle disuse. 2. Establish whether combined β-lactoglobulin supplementation and resistance training for 1 week prior to limb immobilisation will attenuate the decrease in muscle mass and strength during short-term muscle disuse. Hypotheses 1. Combining β-lactoglobulin and resistance training for 1 wk prior to 5 d of limb immobilisation will attenuate the decrease in integrated muscle protein synthesis, muscle fibre CSA, quadriceps muscle strength, and leg lean mass during muscle disuse. 2. Combining β-lactoglobulin and resistance training for 1 wk prior to 5 d of limb immobilisation will attenuate the decrease in muscle fibre CSA, quadriceps muscle strength, and leg lean mass during muscle disuse.
Interventions
DIETARY_SUPPLEMENTBeta-lactoglobulin
Beta-lactoglobulin (BLG) will be administered thrice daily during a 7 day period of prehabilitation and 5 day period of immobilisation when the leg is placed in a brace.
DIETARY_SUPPLEMENTCarbohydrate
Carbohydrate (dextrose monohydrate) will be administered thrice daily during a 7 day period of prehabilitation and 5 day period of immobilisation when the leg is placed in a brace.
Sponsors
University of Nottingham
King's College London
Study design
Allocation
RANDOMIZED
Intervention model
PARALLEL
Primary purpose
PREVENTION
Masking
DOUBLE (Subject, Investigator)
Masking description
Double blinded to supplement (beta-lactoglobulin or carbohydrate)
Intervention model description
This study is a double-blind, 2-arm, randomised placebo-controlled trial
Eligibility
Sex/Gender
ALL
Age
18 Years to 45 Years
Healthy volunteers
Yes
Inclusion criteria
* Male and female * Aged 18-45 years * Healthy * Physically active (≥150-300 minutes of moderate-intensity aerobic physical activity per week OR 75-150 minutes of vigorous-intensity aerobic physical activity per week) * Eumenorrheic and not taking any hormonal birth control (females)
Exclusion criteria
* Dairy allergy or intolerance * Lower limb injury or surgery in the last 6 months, * Lower limb osteoarthritis or other musculoskeletal disorder * A musculoskeletal or blood blotting disorder * An allergy to local anaesthetic * Currently pregnant * Current use of blood thinning medications * Volunteers that take part in structured resistance exercise training * Taking supplements considered to be anabolic to skeletal muscle (protein supplements, creatine, or omega-3 supplements) * Consuming more than 1.2 grams of protein per kilogram of body mass per day in their habitual diet. * Volunteers involved in other studies at the time of enrolment * Volunteers who have taken part in a tracer study in the past 18 months * If without an understanding of verbal or written English * Volunteers with a history of eating disorders
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Muscle protein synthesis | 15 days | The synthesis of amino acids into new muscle protein. |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| Muscle mass | 15 days | Lean body mass will be measured using Dual Energy Xray Absorptiometry. |
Countries
United Kingdom
Contacts
Primary ContactOliver C Witard, PhD
Backup ContactAlix Hughes, MSc
Outcome results
None listed