Biliary Disease
Conditions
Brief summary
Patients admitted to hospital due to obstructive biliary disease secondary to benign and malign etiologies, undergoing ERCP, will be selected. Bile sample will be taken in ERCP procedure with sterile technique. Its macroscopic appearance will be assessed and biliary culture will be performed.
Detailed description
The sample of biliary aspirate will be assessed as follows. 1. Its macroscopic appearance will be analyzed: yellow, black, or purulent. 2. Biliary culture will be performed; if bacterial growth is observed, its identification will be carried out by means of MALDI-TOF MS mass spectrometry and antibiotic susceptibility tests will be carried out, using the automated VITEK system.
Interventions
DIAGNOSTIC_TESTERCP biliary culture
The sample of biliary aspirate will be assessed as follows. 1. Its macroscopic appearance will be analyzed: yellow, black, or purulent. 2. Biliary culture will be performed; if bacterial growth is observed, its identification will be carried out by means of MALDI-TOF MS mass spectrometry and antibiotic susceptibility tests will be carried out, using the automated VITEK system.
Sponsors
Hospital Universitario Dr. Jose E. Gonzalez
Study design
Observational model
COHORT
Time perspective
PROSPECTIVE
Eligibility
Sex/Gender
ALL
Age
18 Years to No maximum
Healthy volunteers
No
Inclusion criteria
* Patients with diagnosis or suspicion of biliary obstruction of any cause * Any gender * Over 18 years of age
Exclusion criteria
* Patients referred and previously treated at another hospital/clinic * Previous ERCP * Pregnancy * Patient refusal to enter the study
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Prevalence of bacterial isolation in the obstructive biliary disease measured by percentage calculation | 1 year | Determine the prevalence by percentage measurement of bacterial isolation in bile fluid in the obstructive biliary disease in patients undergoing ERCP. |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| Bacteria in the obstructed bile duct | 1 year | Isolate and identify most common bacteria that colonize and infect the obstructed bile duct by means of biliary culture and MALDI-TOF MS mass spectrometry, respectively |
| Antibiotic susceptibility of isolated bacteria in bile fluid | 1 year | Determine antibiotic susceptibility of the isolated bacteria in bile fluid of patients with obstructed bile duct by means of the automated VITEK system |
| Bacteria and etiology of obstructive biliary disease | 1 year | Association between isolated bacteria and the etiology of obstructive biliary disease |
Countries
Mexico
Contacts
Primary ContactMarusia González Villarreal
Backup ContactJoel Omar Jáquez Quintana
Outcome results
None listed