Recurrent Pregnancy Loss
Conditions
Brief summary
The overall goals of this proposal are to determine the genetic architecture of recurrent pregnancy loss (RPL) and to discover genomic predictors of RPL.
Detailed description
The following specific aims are proposed: Aim 1: Collect clinically well-characterized samples from two distinct cohorts of participants with unexplained recurrent pregnancy loss (RPL). * Cohort A will consist of trios (product of conception (POC), biological mother, and biological father) with unexplained RPL. Specifically, a cohort of up to 500 rigorously phenotyped trios will be recruited, for which couples' RPL is not attributable to known causes. POC tissues and parental DNA samples (blood or saliva) will be collected. If necessary for the purpose of determining the pathogenicity of sequence variants identified in the trio, collecting DNA samples from other family members after consent will also be considered. The study team may also request DNA or POC tissues from prior pregnancy loss(es) if available. * Cohort B will consist of up to 100 women with a history of three or more pregnancy losses, with or without a liveborn child, and no known etiology. For these participants, DNA samples (blood or saliva) will be collected from the mothers. If high-impact variants are identified, DNA samples from parents or siblings may be collected for segregation analysis after consent. Aim 2: Whole genome sequencing (WGS) will be performed at the Yale Center for Genome Analysis (YCGA), along with bioinformatic analyses to identify pathogenic variants in both cohorts. For Cohort A, analyses will focus on pathogenic variants identified in RPL trios, and for Cohort B, analyses will focus on variants associated with maternal effect genes in the mothers. Pathogenic variants will be comprehensively defined, and fully annotated variant maps will be generated for all included samples to provide the substrate for subsequent novel gene discovery and, ultimately, the development of clinical diagnostic tests.
Interventions
None listed
Sponsors
Yale University
Johns Hopkins University
University of Texas at Austin
Penn State University
Northwestern University
The University of Texas Health Science Center at San Antonio
Wayne State University
Columbia University
University of Colorado, Denver
University of Chicago
Eunice Kennedy Shriver National Institute of Child Health and Human Development (NICHD)
University of Utah
Study design
Observational model
COHORT
Time perspective
PROSPECTIVE
Eligibility
Sex/Gender
ALL
Age
18 Years to 50 Years
Healthy volunteers
Yes
Inclusion criteria
Cohort A - Fetal Intolerome Cohort Inclusion Criteria: * Women with loss of a current singleton pregnancy at \< 20 0/7 weeks gestation, documented by ultrasonography or histopathological examination * History of one or more prior pregnancy losses * Euploid current pregnancy confirmed by karyotype, microarray, or STORK (Short-read Transpore Rapid Karyotyping) sequencing Note: A limited number of aneuploid losses will be included as part of the pilot phase
Exclusion criteria
* History of parental karyotype abnormalities * History of antiphospholipid antibody syndrome * Evidence of uncontrolled diabetes * Evidence of uncontrolled thyroid disease * History of autoimmune disease related to pregnancy loss (e.g., systemic lupus erythematosus, rheumatoid arthritis) * History of uterine anomalies * History of cervical insufficiency Cohort B - Maternal Effect Gene Cohort Inclusion Criteria: \- Women with a history of three or more pregnancy losses of unknown cause, with or without a liveborn child
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Determine genetic etiology for Recurrent Pregnancy Loss | 5 years | The WGS tool will be used to analyze the POC sample and parental blood samples to determine the clinical reportable genetic cause for RPL |
Countries
United States
Contacts
CONTACTYong-Hui Jiang, MD, PhD
CONTACTHeping Zhang, PhD
Outcome results
None listed