Mouth Diseases, Periodontal Diseases, Periodontal Inflammation
Conditions
Brief summary
The aim of this study is; detection of interleukin(IL)-38, IL-36 gamma(γ) , IL-17 and matrix metalloproteinase(MMP)-9 levels in gingival crevicular fluid (GCF), saliva and serum samples of periodontally healthy, gingivitis and periodontitis patients and the possible correlation between these values and clinical parameters of periodontal diseases. Materials and methods: Samples were obtained from 90 systemically healthy non-smoker individuals with periodontitis (P, n=30), gingivitis(G, n=30) and healthy periodontium (S, n=30). Full-mouth clinical periodontal measurements including probing depth (PD), clinical attachment level (CAL), bleeding on probing (BOP), gingival index (GI) and plaque index (PI) were also recorded. Enzyme-linked immunosorbent assay (ELISA) was used to determine IL-38, IL-36γ, IL-17 and MMP-9 levels in the biological samples.
Interventions
DIAGNOSTIC_TESTGCF obtaining
GCF obtaining: 2 GCF samples, 1 from the interproximal surface of 2 different teeth that bleeding on probing(-), were taken from each participant
DIAGNOSTIC_TESTSaliva Obtaining
Saliva obtaining: 1,5 ml unstimulated whole saliva collected from each participant
DIAGNOSTIC_TESTSerum obtaining
Serum obtaining : 8 ml of venous blood was collected from each participant and collecting blood centrifuged to obtain serum
Sponsors
Akdeniz University
Study design
Allocation
NON_RANDOMIZED
Intervention model
PARALLEL
Primary purpose
DIAGNOSTIC
Masking
NONE
Intervention model description
According to full-mouth clinical periodontal measurements including probing depth (PD), clinical attachment level (CAL),bleeding on probing (BOP), gingival index (GI) and plaque index (PI) samples of GCF, saliva and serum were obtained from 90 systemically healthy non-smoker individuals with stage II grade B (P1, n=9), stage III grade B (P2, n=11) , stage III grade C (n=10), gingivitis (G, n=30) and healthy periodontium (S, n=30).
Eligibility
Sex/Gender
ALL
Age
18 Years to 65 Years
Healthy volunteers
Yes
Inclusion criteria
* Systemically healthy (The determination of healthy volunteers will be based on the statements of the patients in the anamnesis. No additional examinations will be made.) * At least twenty permanent teeth in the mouth * Non-smoker * No medication for continuous use * Those who have not used antibiotics, anti-inflammatory and systemic corticosteroid drugs in the last 6 months. * Not in pregnancy or lactation period. * For the periodontitis group that has not received periodontal treatment in the last 6 months
Exclusion criteria
* Any oral or systemic disease * Regularly using a systemic medication * During pregnancy or lactation * Received periodontal treatment within the last 6 months. * Those who received antibiotic, anti-inflammatory or systemic corticosteroid medication in the last 6 months * Smokers
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| Levels of Interleukin-38 (pg/ml) in GCF, saliva and serum fluid | 24 hours after taking the clinical measurements at the first visit |
| Levels of Interleukin-36 gamma (pg/ml) in GCF, saliva and serum fluid | 24 hours after taking the clinical measurements at the first visit |
Secondary
| Measure | Time frame |
|---|---|
| Levels of Interleukin-17 (pg/ml) in GCF, saliva and serum fluid | 24 hours after taking the clinical measurements at the first visit |
| Levels of Matrix Metalloproteinase-9 (ng/ml) in GCF, saliva and serum fluid | 24 hours after taking the clinical measurements at the first visit |
Countries
Turkey (Türkiye)
Outcome results
None listed