Gestational Diabetes Mellitus in Pregnancy, Obesity, Maternal
Conditions
Brief summary
Aim of the study was to determine if signaling pathways in placentas of mothers affected by overweight/obesity or by gestational diabetes are altered compared to placentas of a control group (normal weight, no gestational diabetes). Moreover, maternal blood and umbilical cord blood were analysed.
Interventions
OTHERno intervention
no intervention
Sponsors
Sarah Appel
Study design
Observational model
COHORT
Time perspective
RETROSPECTIVE
Eligibility
Sex/Gender
FEMALE
Age
18 Years to No maximum
Inclusion criteria
* singleton pregnancy * BMI before pregnancy \>= 18 * with & without gestational diabetes * c-section at gestational week 35+0 until 41+6
Exclusion criteria
* multiple pregnancy * maternal serious internal disease * infections before pregnancy (e.g. HIV, hepatitis B) * pregnancy-associates diseases (e.g. gestoses, androgen insensitivity syndrome)
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Activity of Wnt signaling pathway in placental tissue | through study completion, about 4 years in total | Analysis of components of the Wnt signaling pathway; like LRP6 receptor, beta-Catenin, GSK3beta, Wnt ligands (=proteins of interest) and an appropriate protein for normalization. Total protein lysates of placental tissue will be used. Analysis will be performed by western blotting and subsequent densitometric analysis. Outcome measure will be \[relative protein level of protein or interest/relative protein level of normalization protein\]. |
Countries
Germany
Outcome results
None listed