Bariatric Surgery Candidate, Digestive Organs--Cancer, Parietal Region Trauma
Conditions
Keywords
Mitochondria, Oxygraphy, Adipose Tissue, Body Mass Index, Digestive surgery
Brief summary
The objective of this study is to adapt the oxygraphy technique on human adipocytes and to characterize respiration measurements according to patients' body mass index and white adipose tissue localization (subcutaneous vs visceral).
Detailed description
During digestive surgery: oncological and bariatric surgery, it is possible to collect a small volume of subcutaneous and epiplonic adipose tissue (10-20 cc). We wish to collect these samples in order to develop the technique of oxygenography on permeabilized adipocytes. In order to characterize these measurements, they will be related to the clinical data of the patients usually collected in the framework of the preoperative assessment including the body mass index (BMI). The measured adipocyte data will thus be normalized to three BMI classes: ≤ 20, \> to 20 \< 30, and ≥ to 30 kg∙m-2. Primary end point is to validate the measurement of mitochondrial respiration under oxidophosphorylating conditions by determining indicators of the distribution of mitochondrial respiration values (mean, standard deviation, skewness, kurtosis, coefficient of variation) as a function of patients' body mass index.
Interventions
PROCEDUREadipose surgery
A quantity of adipose tissue equivalent to 10-20 cubic centimeter will be removed subcutaneously and from the omentum during surgical procedures (oncological, bariatric)
Sponsors
University Hospital, Clermont-Ferrand
Study design
Allocation
NA
Intervention model
SINGLE_GROUP
Primary purpose
HEALTH_SERVICES_RESEARCH
Masking
NONE
Intervention model description
all participants receive the same intervention
Eligibility
Sex/Gender
ALL
Age
18 Years to No maximum
Healthy volunteers
No
Inclusion criteria
* Patients undergoing oncological, bariatric, parietal surgery * Patients able to give an informed consent * Patients affiliated to an Health Care insurance * Patients aged over 18
Exclusion criteria
* Patients under guardianship, curatorship or deprived of freedom * Pregnant or breastfeeding women * Participation refusal * Patients under chemotherapy * Patients with peritonitis and infectious parietal processes
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Evaluation of mitochondrial respiration on adipose tissue by oxygraphy | day 1 | Mitochondrial respiration measurements in oxydophosphorylated conditions. For each patient measurements using ADP and carbohydrate or lipid substrates of the respiratory chain complexes for both the subcutaneous and the visceral tissue. |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| mitochondrial respiration values depending on patients BMI by oxygraphy | day 1 | Identification of mitochondrial respiration discrimination factors |
| comparing mitochondrial respiration values depending on tissue location by oxygraphy | day 1 | Identification of mitochondrial respiration discrimination factors |
| comparing mitochondrial respiration values depending on anthropometric data by oxygraphy | day 1 | Identification of mitochondrial respiration discrimination factors |
Countries
France
Contacts
PRINCIPAL_INVESTIGATORRuddy RICHARD
University Hospital, Clermont-Ferrand
Outcome results
None listed