Amino Acid, Body Composition, Exercise, Probiotic
Conditions
Brief summary
The most common problem among sports people is that no matter how much protein food or products they eat, their sports performance cannot be improved, resulting in failure to achieve breakthroughs in sports performance. The cause of the trouble is digestion and absorption problems. The key is intestinal problems. If gastrointestinal function is maintained in a healthy state, then exercise performance must be maintained at a considerable level. Therefore, how to choose the source of protein in the diet is one of the most concerned issues of the sports crowd. Although past studies have confirmed that the essential amino acids in animal protein can be absorbed and utilized better than plant protein, plant protein can be broken down into easily digestible peptides and amino acids by pepsin. Promote the metabolite pool in the large intestine and the amino acid balance of the host in the small intestine. Recent studies have pointed out that the proteases and peptidases in lactic acid bacteria can provide free amino acids for the best growth of bacteria, and can increase the distribution of amino acids in the blood, the speed of muscle synthesis and the content of branched chain amino acids. However, the mechanism of action of Lactobacillus plantarum on protein digestion and amino acid absorption in the host is still unclear. Therefore, the purpose of this study is to explore the effect of supplementing sports lactic acid bacteria TWK10 in human experiments to effectively improve the amino acid bioabsorption rate of plant protein supplements. 40 subjects were randomly divided into 2 groups, each with 20 people (male and female): (1) pea protein without TWK10 (placebo), (2) TWK10 group (TWK10). The two groups of subjects were supplemented with test samples for 28 consecutive days and performed paired sports training 3 times a week. Before and after the intervention, the samples were subjected to exercise testing, blood and fecal sample collection, body composition analysis, and muscle mass analysis.
Interventions
DIETARY_SUPPLEMENTTWK10
1. 4 days without training: drink 2 hours before bedtime. 2. 3 days of training: drink within 30 minutes after training (uniformly brewed in the laboratory). 3. To analyze the amino acid concentration in blood, drink it immediately before blood collection.
OTHERPlacebo
1. 4 days without training: drink 2 hours before bedtime. 2. 3 days of training: drink within 30 minutes after training (uniformly brewed in the laboratory). 3. To analyze the amino acid concentration in blood, drink it immediately before blood collection.
Sponsors
Synbio Tech Inc.
Chi-Chang Huang
Study design
Allocation
RANDOMIZED
Intervention model
PARALLEL
Primary purpose
BASIC_SCIENCE
Masking
DOUBLE (Subject, Investigator)
Eligibility
Sex/Gender
ALL
Age
20 Years to 45 Years
Healthy volunteers
Yes
Inclusion criteria
* Have exercise habits (exercise at least 3 times a week for at least 30 minutes each time), * Healthy body
Exclusion criteria
* Smoking * Alcohol consumption * Cardiovascular disease * Neuromuscular disease * Metabolic disease * Asthma, pregnant or breastfeeding * BMI\>27 * Allergic to peanuts .
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Bench press test | From Baseline to 4 Weeks Assessed | Bench press with Smith machine and add weight to find individual 1RM |
| Amino acid | From Baseline to 4 Weeks Assessed, including time point: baseline, 30 minutes, 60 minutes, 120 minutes, and 180 minutes post ingestion) | Liquid chromatography tandem mass spectrometry (LC-MS/MS) was used for analysis, wherein the liquid chromatography system was Acquity UPLC, and the column used was Waters C18 column (2.1 × 150 mm, 1.8 μm), and the temperature of the column was set at 45 °C, the mobile phases used were water and 100% acetonitrile, both of which contained 0.1% formic acid, the flow rate was 0.6 mL/min, and the injection volume of the test solution was 2 μL. |
| Isometric Mid-Thigh Pull (IMTP) | From Baseline to 4 Weeks Assessed | Customized IMTP test equipment and two force plates (type 9287BA, Kistler Instruments AG, Winterthur, Switzerland) were used. All participants stood with their feet the same width apart, and the rod was placed between the thighs, with the torso upright, the spine neutral, and the knee and hip angles at 140°, to familiarize the participants with the IMPT test method. The average absolute peak force (PF) were recorded. |
| Wingate Anaerobic Test (WAnT) | From Baseline to 4 Weeks Assessed | After a standard warm-up, all subjects were assessed with the classical WAnT on a cycloergometer (Monark 894E, Varberg, Sweden) in a 30 s go all out ultramax test. The seat height was adjusted to the satisfaction of each participant, and toe clips prevented the feet from slipping off the pedals. Before the initial test, the subjects warmed up for 5 min, and the power was approximately 50 W. After the warm-up, two preparation exercises lasting 3 s, during which the actual test load was 3% of their own body weight, were applied to accustom the participant to resistance. The test started, and the resistance was set on the friction belt of the dynamometer. External loading was estimated individually at 5% body weight. The recorded results were the relative mean power (W/kg) |
| dual-energy X-ray absorptiometry, DXA | From Baseline to 4 Weeks Assessed | A non-invasive dual-energy X-ray absorptive bone density testing room (Lunar iDXA, GE Healthcare, Chicago, IL, USA) was used for systemic body composition and bone density measurements. The subject was required to lie flat on the test bed, with their body at the center line, and their limbs within the detection range. Two different energy X-rays were used to scan the inspected part, then the scintillation detector received the X-rays that had penetrated the inspected part, and analyzed the obtained muscle mass |
| Muscle composition | From Baseline to 4 Weeks Assessed | Use muscular ultrasound (BenQ T3300) to scan |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| Checklist Individual Strength (CIS) questionnaire | From Baseline to 4 Weeks Assessed | Assessing subjects' living conditions using the CIS scale. 1 is the least match, 5 is the most match |
| Visual Analog Fatigue Scale (VAFS) | From Baseline to 4 Weeks Assessed | Using the VAS scale to measure the subjective feelings of the subjects after exercise, 1 is the least tired, 5 is the most tired |
| Rating of Perceived Exertion (RPE) scale | From Baseline to 4 Weeks Assessed | Use the RPE scale to record how hard the subjects are after the test, 6 points are the least hard, 20 points are the hardest |
| 3-day dietary recording four weeks | From Baseline to 4 Weeks Assessed | After 4 weeks of intervention, the 3-day diet was recorded and the calorie intake was calculated by the dietitian |
Countries
Taiwan
Outcome results
None listed