Exercise, Healthy Male/Female Subjects
Conditions
Keywords
Ketone, Inactivity-induced muscle atrophy, Retraining-induced regeneration
Brief summary
The aim of this study is to investigate the effect of oral ketone administration during inactivity-induced muscle atrophy and retraining-induced regeneration. Potential changes in muscle function (cycling performance, knee-extension force and power, jump height) and mass, blood flow and the muscular extracellular matrix will be the main focus. In this context, the dominant leg of the participants (n=24) will be immobilized with a brace for 2 weeks and subsequently retrained in a 4-week progressive resistance exercise training program to stimulate muscle regeneration. During the immobilization and rehabilitation period, participants receive either ketone ester (KE) or placebo (CON). 4 experimental sessions are performed during this time frame in order to evaluate the effects of the trial.
Interventions
DIETARY_SUPPLEMENTMedium Chain Triglyceride (MCT) oil
A daily dose of 3x12.8g of MCT oil supplementation during a six week intervention period
DIETARY_SUPPLEMENTKetone ester
A daily dose of 3x20g of ketone ester supplementation during a six week intervention period
Sponsors
KU Leuven
Study design
Allocation
RANDOMIZED
Intervention model
PARALLEL
Primary purpose
TREATMENT
Masking
QUADRUPLE (Subject, Caregiver, Investigator, Outcomes Assessor)
Eligibility
Sex/Gender
ALL
Age
18 Years to 35 Years
Healthy volunteers
Yes
Inclusion criteria
* Recreational sports participation, including general fitness training, between 2 and 5 hours max per week * Good health status confirmed by a medical screening * Body mass index between 18 and 25
Exclusion criteria
* Any kind of injury/pathology that is a contra-indication to perform resistance exercise * Intake of any medication or nutritional supplement that could impact muscle protein synthesis during the period of the study * Intake of any whey protein, casein or branched-chain amino acid supplement or anti-inflammatory drug from 1 month prior to the start of the study * Blood donation within 3 months prior to the start of the study * Smoking * Pregnant * More than 3 alcoholic beverages per day * Current participation in another research trial * Any other argument to believe that the subject is unlikely to successfully complete the full study protocol * Adherence to a high-fat, low-carbohydrate ketogenic diet (less than 20% of energy intake derived from carbohydrates) * (Cow's) milk protein allergy
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Exercise performance | Day 0 - day 14 - day 28 - day 42 | Changes in exercise performance as evaluated during a graded exercise test |
| Echo-Doppler measurement of femoral artery | Day 0 - Day 14 - Day 28 - Day 42 | Change in blood flow of arteria femoralis |
| Muscle volume | Day 0 - Day 14 - Day 28 - Day 42 | Change in volume of quadriceps evaluated by computed-tomography scan |
| Muscular functional capacity | Day 0 - Day 14 - Day 28 - Day 42 | Change in mean power produced during 30 unilateral knee extensions on a knee-extension apparatus |
| Skeletal muscle biopsies | Day 0 - Day 14 - Day 28 - Day 42 | Changes in myofibrillar protein synthesis |
| Blood marker of collagen synthesis | Day 0 - Day 14 - Day 28 - Day 42 | Change in PINP (n-terminal peptide of pro-collagen I) concentration in blood |
Countries
Belgium
Contacts
Primary ContactPeter Hespel, Prof.
Backup ContactRuben Robberechts
Outcome results
None listed