Healthy, Nutrition, Healthy
Conditions
Brief summary
The aim of this study is to investigate the non-heme iron absorption from single meals of texturized fava bean protein, which has been suggested as a protein source suitable for replacement of meat, compared to the non-heme iron absorption from beef protein and cod protein meals. The study participants are women of fertile ages, which is one of the main groups at risk of developing iron deficiency. Since a dietary shift towards increasingly plant based is being pushed, investigating nutritional effects of such a shift is essential to avoid potential negative effects such as an increase of nutritional deficiencies. The study includes two cross-over single-blinded iron isotope trials in apparent healthy Swedish women of the ages 18-45 years, each of whom served as their own control. Participants will be served matched test meals containing beef and fava bean protein (Study 1), or cod and fava bean protein (Study 2) with radiolabeled non-heme iron 55Fe and 59Fe. Absorption of non-heme iron from test meals will be measured by whole-body counting, and erythrocyte incorporation by liquid scintillation.
Interventions
OTHERSingle meal non-heme iron absorption
Two study groups, each designed as cross-over single-blinded iron isotope trials in apparent healthy Swedish women of the ages 18-45 years. Each participant serves as their own control. Meals are tagged with radiolabeled non-heme iron 55Fe and 59Fe.
Sponsors
Göteborg University
Chalmers University of Technology
Study design
Allocation
RANDOMIZED
Intervention model
CROSSOVER
Primary purpose
OTHER
Masking
SINGLE (Subject)
Masking description
Meals are served from identical plastic boxes. Participants are served the meals in a separate room from preparation
Eligibility
Sex/Gender
FEMALE
Age
18 Years to 45 Years
Healthy volunteers
Yes
Inclusion criteria
* Female * Age 18-45 * Is tolerant to intake of fava bean * Eats fish and meat * Feels healthy * Can eat a breakfast consisting of three potato-based patties
Exclusion criteria
* Other sex than female * Age \<18 and \>45 * Has an ongoing infection * Is vegetarian * Is pregnant or lactating * Takes iron supplements
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| 59Fe non-heme iron absorption from cod and beef protein meals | 5 weeks | Absorption of 59Fe, which is added to the animal protein meals, will be measured by a whole body counter which enables measurement of absolute value. |
| 55Fe non-heme iron absorption from fava bean protein meals. | 5 weeks | The absorption quota of 59Fe/55Fe is measured by erythrocyte incorporation, analyzed by liquid scintillation. The absorbance quota, in combination with the absolute value of the whole body counter, will be used to calculate the absorbance of 55Fe which is added to the fava bean meals |
Countries
Sweden
Outcome results
None listed