Prevention & Control
Conditions
Keywords
Mouth rinses, substanticity, oral infections, salivary microbiota
Brief summary
A desirable quality of mouthrinses is substantivity an intrinsic feature of some molecules. Substantivity requires the adsorption of the agent on the oral surfaces and subsequently antimicrobial activity bacteriostatic along following hours. This work aims to quantitatively determine the substantivity of (i) a single o-Cymen-5-ol plus CPC oral rinse, (ii) a, CPC oral rinse and (iii) a o-Cymen-5-ol mouthwash on the salivary microbiota up to 4 h after their application.
Detailed description
One of the desirable qualities of mouthrinses is substantivity, which is defined as the persistence capacity of its antimicrobial action over time in the mouth. This is an intrinsic feature of some molecules. Such substantivity seems to require the adsorption of the agent on the buccal surfaces and subsequently exert a certain antimicrobial activity that seems to be mostly bacteriostatic. Substantivity is the result of multiple factors controlling or altering adhesion, life period of antimicrobial capabilities, synergisms, antagonisms, and others. In order to improve substantivity and therefore increase antimicrobial effectiveness of CPC, o-Cymen-5-ol has recently been proposed to become incorporated to oral rinses. The aim of this work is to quantitatively determine the substantivity of (i) a single o-Cymen-5-ol plus CPC oral rinse, (ii) a, CPC oral rinse and (iii) a o-Cymen-5-ol mouthwash on the salivary microbiota up to 4 h after their application.
Interventions
OTHEROral rinse
Participants use mouthwasher for a given time.
OTHERSaliva harvest
Non-stimulated saliva harvesting
Sponsors
University of Barcelona
Study design
Allocation
RANDOMIZED
Intervention model
CROSSOVER
Primary purpose
PREVENTION
Masking
NONE
Intervention model description
randomized, double-blind, crossover study of the in situ antibacterial efficacy of o-Cymen-5-ol plus CPC, o-Cymen-5-ol and CPC on salivary microbiota.
Eligibility
Sex/Gender
ALL
Age
18 Years to 45 Years
Healthy volunteers
Yes
Inclusion criteria
1. adult age (\>18 y/o) 2. good oral helath status 3. minimum 24 evaluable teeth 4. periodontal pockets ≤3 mm 5. absence of caries & periodontal haemorrage
Exclusion criteria
1. smokers 2. prosthesis or orthodontic devices 3. Sjögren syndrome 4. Antibiotic treatment in the last three months 5. Systemic disease with alteration in the production and/or composition of the saliva
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Preliminary experiments | one month | Measure of surviving bacteria by fluorimetry and confocal microscopy (Live & dead) |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| Substantivity of mouthrinse formulations containing Cetylpyridinium Chloride and O-cymen-5-Ol (single and combined) compared to Placebo on salivary microbiota | up to three months | Measure of surviving bacteria by fluorimetry and confocal microscopy (Live & dead) rinses plus placebo |
Countries
Spain
Outcome results
None listed