Cellular Responses Within Skeletal Muscle in Response to Exercise of Different Intensities, Changes Within Adipose Tissue in Response to Exercise at the Three Different Intensities, Relationship Between the Cellular Responses in Skeletal Muscle and the Changes in the Metabolomic Profile
Conditions
Brief summary
The study will assess the exercise-induced changes in chromatin accessibility and gene expression in a cell type-specific manner in skeletal muscle from healthy adults in response to exercise at three different intensities - 1) low-intensity continuous exercise, 2) moderate-intensity continuous exercise, and 3) high-intensity interval exercise.
Interventions
BEHAVIORALLow intensity exercise session
subjects will exercise at 30% maximal oxygen consumption (VO2max) for 45-60 minutes
BEHAVIORALModerate intensity exercise session
subjects will exercise at 65% maximal oxygen consumption (VO2max) for 30-45 minutes
BEHAVIORALHigh intensity exercise session
subjects will completed 10x1minute exercise intervals at maximal oxygen consumption (VO2max) with 1 minute active recovery between
Sponsors
University of Michigan
Study design
Allocation
RANDOMIZED
Intervention model
PARALLEL
Primary purpose
BASIC_SCIENCE
Masking
NONE
Eligibility
Sex/Gender
ALL
Age
18 Years to 40 Years
Healthy volunteers
Yes
Inclusion criteria
* Age:18-40 * Body Mass Index: 20-30 kg/m2 * Must engage in regularly planned aerobic-type exercise at moderate-vigorous intensity, 3 times per week for at least 30-60 minutes/session * Women must have regularly occurring menses and must be premenopausal * Subject willingness to opt-in for the Michigan Genomics Initiative (MGI) Repository
Exclusion criteria
* Evidence/history of cardiovascular or metabolic disease. * Taking anti-coagulate medications or medications known to affect metabolism or inflammation * Weight instability ≥ ±3kg in the last 6 months * Tobacco or e-cigarette users * Women must not be pregnant or actively lactating
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| post-translational modifications of skeletal muscle oxidative enzymes | 30 minutes before exercise | Western blot |
| post-translational modifications of Adipose tissue metabolic proteins | 15 minutes before exercise | Western blot |
| mRNA expression of skeletal muscle oxidative enzymes | 30 minutes before exercise | qPCR |
| mRNA expression of adipose tissue metabolic enzymes | 15 minutes before exercise | qPCR |
Countries
United States
Outcome results
None listed