Dehydration, Cognitive Change, Gastrointestinal Microbiota
Conditions
Brief summary
The central hypothesis is that improving hydration through increased water consumption will change the relative abundance of mucolytic bacteria found in the stool. Therefore the specific aims are 1) to quantify intervention effects on fecal microbiota relative abundance and plasma lipopolysaccharide binding protein, 2) observe the effects of the intervention on bowel frequency and signs/symptoms of gastrointestinal stress, and 3) to investigate relations between executive function and hydration status.
Detailed description
A single arm 3-week hydration intervention will be employed where participants increase their water consumption to 2 (F) or 2.5(M) liters per day which is approximately 70% of the AI for daily water consumption. Pre-test and follow-up measures of fecal microbiota, urinary hydration status, cognitive function, circulating markers, and dietary intake will be assessed at baseline and at 3-week follow up via laboratory visits.
Interventions
DIETARY_SUPPLEMENTWater Intake
Participants will increase plain water consumption to at least 70% of the daily adequate intake for Americans depending on their sex.
Sponsors
Division of Nutritional Sciences, University of Illinois at Urbana-Champaign
University of Illinois at Urbana-Champaign
Study design
Allocation
NA
Intervention model
SINGLE_GROUP
Primary purpose
PREVENTION
Masking
NONE
Intervention model description
Single arm hydration water intake intervention with a baseline urine concentration threshold for eligibility
Eligibility
Sex/Gender
ALL
Age
19 Years to 50 Years
Healthy volunteers
Yes
Inclusion criteria
* 19-50 years of age * 18.5-34.49 kg/m2 * 24-hour UOsm above 500 mOsm/kg * No antibiotic use over the past 3 months * Absence of metabolic diseases and use of diuretics * Agree to maintain typical diet intake (e.g., dietary fiber) patterns during intervention * Avoid consuming prebiotic and probiotic supplements during study participation * Not pregnant * Agree to follow the study protocol
Exclusion criteria
* \<19 or \>50 years of age * \<18.5 or \>34.49 kg/m2 * 24-hour UOsm \<500 mOsm/kg * Antibiotic use over the past 3 months * Metabolic diseases and use of diuretics * Not agree to maintain typical diet intake (e.g., dietary fiber) patterns for the duration of the * intervention * Not agree with avoiding consuming prebiotic and probiotic supplements during study participation * Pregnant * Not agree to follow study protocol
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Fecal microbiota relative abundance | 3 weeks (baseline vs. follow-up) | changes in the relative abundance of fecal microbiota |
| Plasma lipopolysaccharide (LPS) | 3 weeks (baseline vs. follow-up) | changes in circulating LPS |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| Attentional accuracy | 3 weeks (baseline vs. follow-up) | Accuracy (%) on a computerized flanker task |
| Attentional Reaction Time | 3 weeks (baseline vs. follow-up) | Reaction time (ms) on a computerized flanker task |
| Copeptin | 3 weeks (baseline vs. follow-up) | changes in plasma copeptin concentration |
| 24hr Urine Specific Gravity | 3 weeks (baseline vs. follow-up) | changes in specific gravity (USG) of urine samples |
| Attentional processing speed | 3 weeks (baseline vs. follow-up) | P3 event related potential latency (ms) using a computerized flanker task |
| 24hr Urine Osmolality | 3 weeks (baseline vs. follow-up) | changes in osmolality (mOsmol/kg) of urine samples |
Countries
United States
Outcome results
None listed