Energy Balance, Gut Hormones, Metabolic Health
Conditions
Keywords
GLP-1, PYY, Breaking sitting, Energy balance, Postprandial glycaemia
Brief summary
The purpose of this project is to investigate the effects of breaking up prolonged sitting on postprandial metabolic responses, gut hormones secretion and energy balance in sedentary overweight and obese adults.
Detailed description
Two trials are included in this project - Prolonged sitting and Breaking sitting. In the Prolonged sitting trial, after consuming breakfast, participants sat on a chair for 180 min. After 180 min, lunch was provided. After finishing lunch, participants sat on a chair for another 120 min. In the Breaking sitting trial, after consuming breakfast, participants walked 2 min on a treadmill every 20 minutes for the following 180 min. For the remainder of the time participants sat on the chair. After 180 min, lunch was provided. After finishing lunch, participants continued 2 min of walking every 20 min for the following 120 min. Blood samples were collected in a regular pattern throughout the trials. Energy balance (e.g., energy intake and spontaneous physical activity) was assessed using food diary and an accelerometer until midnight in the trial days once participants left laboratory.
Interventions
BEHAVIORALProlonged sitting
After consuming breakfast, participants sat on a chair for 180 min. After 180 min, lunch was provided. After finishing lunch, participants sat on a chair for another 120 min.
BEHAVIORALBreaking sitting
After consuming breakfast, participants walked 2 min on a treadmill every 20 minutes for the following 180 min. For the remainder of the time participants sat on the chair. After 180 min, lunch was provided. After finishing lunch, participants continued 2 min of walking every 20 min for the following 120 min.
Sponsors
National Taiwan Normal University
Study design
Allocation
RANDOMIZED
Intervention model
CROSSOVER
Primary purpose
BASIC_SCIENCE
Masking
NONE
Eligibility
Sex/Gender
ALL
Age
20 Years to 45 Years
Healthy volunteers
Yes
Inclusion criteria
* Physical inactive males and females * Aged between 20 and 45 years * Overweight and obese healthy adults (waist circumference greater than 90 cm for males and 80 cm for females) * Weight stable for more than 3 months (no change in weight +/- 3%) * Non-smoker * Able to walk comfortably on a treadmill
Exclusion criteria
* Personal history of/existing diabetes, cardiovascular disease, metabolic disease or dyslipidaemia * Waist circumference \< 90 cm for male and \< 80 cm for female * Taking medications that may influence lipid or carbohydrate metabolism or immune system function * Unable to take part in exercise for any reason (e.g., injury or disability) or a positive response to any questions on the Physical Activity Readiness questionnaire (PAR-Q)
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Gut hormones responses | 320 minutes | Changes in blood gut hormones responses (e.g., GLP-1, PYY and GIP concentrations measured in pmol/L) between trials |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| Energy intake | 600 minutes after leaving the labalotory | Changes in energy intake (e.g, using a food diary to record) after leaving the laboratory until midnight |
| Physical activity level | 600 minutes after leaving the labalotory | Changes in physical activity level (e.g., using an accelerometer to record) after leaving the laboratory until midnight |
| Lipid profile | 320 minutes | Changes in blood lipid profile (e.g., TAG and cholesterol, etc.) between trials |
| Visual analogue scale (VAS) | 320 minutes | Changes in VAS from 0-100 mm (e.g., appetite and mood, etc.) between trials |
| Computerized cognitive testing | 320 minutes | Changes in cognitive function (e.g., accuracy and reaction time) between trials |
| Incremental area under curve (iAUC) for metabolic health | 320 minutes | Changes in incremental area under curve (iAUC) for insulin and glucose between trials |
| Blood pressure | 320 minutes | Changes in blood pressure (e.g., diastolic and systolic blood pressure) between trials |
Countries
Taiwan
Outcome results
None listed