Vitiligo
Conditions
Brief summary
The purpose of this clinical trial is to study the effect of Narrow Band UVB on E-cadherin and integrin alpha v beta 1 as representatives of adhesion molecules in non-segmental vitiligo in a trial to verify a novel proposed mechanism of Narrow Band UVB. Additionally, specific melanocyte marker (Melan A) will be done to settle the localization of adhesion molecules in relation to melanocytes.
Interventions
DEVICENarrow band UVB
Narrow band UVB emitting UVB at wavelength 311nm
Sponsors
Kasr El Aini Hospital
Study design
Allocation
NA
Intervention model
SEQUENTIAL
Primary purpose
TREATMENT
Masking
NONE
Eligibility
Sex/Gender
ALL
Age
18 Years to 60 Years
Healthy volunteers
Yes
Inclusion criteria
* All clinically diagnosed cases of non-segmental vitiligo . * Patients with VIDA +1 or more (new/expanding lesions active in time period of 6-12 months ago) will be included in the trial. Activity of vitiligo will be evaluated through Vitiligo Disease Activity (VIDA) Score.
Exclusion criteria
* Patients who have stable disease at the time of study i.e. no progression of existing disease or appearance of new lesions in the last six months. * Patients receiving treatment for vitiligo 3 months prior to the study. * Patients with contraindications for phototherapy. * Patients with current/history of any systemic and/or dermatological diseases affecting the immune system as autoimmune diseases and malignancy.
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Melanocyte marker (Melan A) for staining of melanocytes | 4 months | Immunohistochemistry and image analysis Data were obtained using Leica Quinn software with Olympus BX40 microscope and Panasonic camera Measurements were taken in 5 non-overlapping high power fields. |
| E-cadherin expression as a cell adhesion molecule between melanocytes and keratinocytes | 4 months | Immunohistochemistry and image analysis Data were obtained using Leica Quinn software with Olympus BX40 microscope and Panasonic camera Measurements were taken in 5 non-overlapping high power fields. |
| Integrin alpha v beta 1 expression as a cell adhesion molecule between melanocytes and keratinocytes | 4 months | Immunohistochemistry and image analysis Data were obtained using Leica Quinn software with Olympus BX40 microscope and Panasonic camera Measurements were taken in 5 non-overlapping high power fields. |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| Vitiligo Area and severity index (VASI) | 4 months | Minimum value is 0, maximum value is 100 Higher scores mean worse outcome |
Other
| Measure | Time frame | Description |
|---|---|---|
| Vitiligo Disease Activity score (VIDA) | 4 months | Minimum value -1, maximum value 4 Higher scores mean worse outcome |
Countries
Egypt
Outcome results
None listed