Red Blood Cell Disorder
Conditions
Keywords
blood preservation solution III, intraoperative blood salvage
Brief summary
To evaluate the effects of blood preservation solution III (sodium citrate, sodium dihydrogen phosphate, sodium citrate, glucose and adenine) in autologous blood recovery and storage bags on the metabolic morphological functions and characteristics of red blood cells in patients undergoing cardiac surgery supported by cardiopulmonary bypass
Detailed description
Blood preservation solution III is a blood protective solution clinically used to store whole blood and red blood cells. It is a sterilized aqueous solution composed of sodium citrate dihydrogen phosphate sodium citrate glucose and adenine, in which glucose adenine can provide the required energy for stored RBC. Citric acid/sodium citrate buffer pair can adjust the pH of RBC suspension; Sodium dihydrogen phosphate can prevent RBC aggregation, provide phosphate for RBC energy metabolism, and slow down the decline rate of 2,3-DPG. Blood preservation solution III can reduce RBC destruction and protect its function. Therefore, this study aims to explore the effect of adding blood preservation solution III in blood storage bags on CPB Influence of autologous blood recovery RBC during cardiac surgery.
Interventions
COMBINATION_PRODUCTblood preservation solution III
Blood bag added blood preservation solution III
Sponsors
Second Affiliated Hospital, School of Medicine, Zhejiang University
Study design
Allocation
RANDOMIZED
Intervention model
PARALLEL
Primary purpose
OTHER
Masking
SINGLE (Subject)
Eligibility
Sex/Gender
ALL
Healthy volunteers
No
Inclusion criteria
Elective median incision for cardiac surgery under CPB ASAⅠ\ Ⅲ Intraoperative blood recovery is pollution-free No blood system diseases, no red blood cell related diseases Preoperative blood routine examination showed no obvious abnormalities in blood coagulation function and biochemistry
Exclusion criteria
* Patients have malignant tumor Patients are complicated with systemic infectious disease Intraoperative blood loss is expected to be less than 200ml Patients receiving allogeneic red blood cells during operation
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| hemoglobin and free hemoglobin concentrations | up to 48 hours | hemoglobin and free hemoglobin concentrations in both groups |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| RBC morphology | autologous blood transfusion immediately, 24 hours, 48 hours | erythrocyte morphology was observed by Rayner's staining and examined under microscope |
| Adenosine acid (ATP ADP AMP) content in RBC | autologous blood transfusion immediately, 24 hours, 48 hours | the clarified supernatants were used for ATP, ADP, and AMP measurements with kits |
| 2, 3-diphosphate (2,3-DPG) concentration | autologous blood transfusion immediately, 24 hours, 48 hours | the cellular 2,3-DPG was analyzed fluorometrically with a kit |
Countries
China
Contacts
Primary ContactMingxia Liu
Backup ContactMin Yan
Outcome results
None listed