Microchimeric Cell Tranfer From Mother to Child - Pilo Study

Identifying and Quantifying Microchimeric Maternal Cells in Young Males, Potentially Acquired Through Breastfeeding - A Pilot Study

Status

UNKNOWN

Phases

Unknown

Study type

Observational

Source

ClinicalTrials.gov

Registry ID

NCT05250219

Enrollment

Registered

2022-02-22

Start date

2021-09-01

Completion date

2022-12-31

Last updated

2022-02-22

For informational purposes only — not medical advice. Sourced from public registries and may not reflect the latest updates. Terms

Conditions

Breastfeeding

Keywords

Microchimeric, Microchimerism

Brief summary

Microchimeric cells are known to be transferred from mother to child through the placenta. However it has been postulated that they could also be transferred through breastfeeding. This study is designed to analyse lymphoid and digestive tract tissues (tonsils and appendixes) in male infants (or young adults) to research microchimeric female cells and try to correlate the frequency and localisation of said cells to the breastfeeding status during early infancy

Interventions

None listed

Study design

Observational model

COHORT

Time perspective

RETROSPECTIVE

Eligibility

Sex/Gender

MALE

Age

1 Years to 16 Years

Healthy volunteers

Inclusion criteria

* age 1-16 years (at the time of surgery) * male * known breastfeeding status in infancy

Exclusion criteria

* premature birth (before 37 weeks) * history of blood transfusion * history of bone marrow transplant * history of organ transplant * patient known to have a twin * known gonosomal

Design outcomes

Primary

Measure	Time frame	Description
To identify microchimeric cells of maternal origin in lymphoid	day 1	To identify microchimeric cells of maternal origin in lymphoid in young males cytogenetic FISH staining, coupled with MS-PCR, to identify microchimeric cells.
To identify microchimeric cells of maternal origin in digestive tissues	day 1	To identify microchimeric cells of maternal origin in digestive tissues in young males cytogenetic FISH staining, coupled with MS-PCR, to identify microchimeric cells.
to determine Quantifying the microchimeric cells	day 1	Quantifying the microchimeric cells : Cell quantification will be assessed through FISH staining (automated cell counting) and qPCR
to determine locating the cells in tissues	day 1	locating the cells in tissues : Appendix histology slides will be analysed after FISH staining to locate the microchimeric cells
to determine trying to find a correlation between breastfeeding status	day 1	trying to find a correlation between breastfeeding status : Microchimeric cell quantification will be confronted to the breastfeeding status in infancy (collected during patient inclusion) to validate a correlation

Countries

France

Contacts

Primary ContactFranck PELLESTOR, MD PHD

f-pellestor@chu-montpellier.fr06.29.04.83.18

Backup ContactBoris DMITRENKO, interne

b-dmitrenko@chu-montpellier.fr06.29.04.83.18

Outcome results