Aging, Clonal Hematopoiesis of Indeterminate Potential, Hip Fractures, Osteoarthritis, Hip, Osteoporosis
Conditions
Brief summary
Bone marrow samples will be collected from patients undergoing hip arthroplasty surgery. Blood and bone marrow samples will be used for metabolic profiling and analysis of relevant CHIP mutations. Combined single-cell transcriptomics and mutation-specific single-cell genotyping (biotin-PCR using mutation-targeted primers followed by sequencing) will subsequently be performed. The gene expression profile of wildtype and mutant hematopoietic stem cells will be compared, performing both broad gene set enrichment analysis and targeted analysis of metabolic pathways.
Interventions
OTHERBlood and bone marrow cell collection
Collection of blood and bone marrow
Sponsors
Imelda Hospital, Bonheiden
Université Catholique de Louvain
Study design
Observational model
CASE_CONTROL
Time perspective
PROSPECTIVE
Eligibility
Sex/Gender
ALL
Age
55 Years to No maximum
Healthy volunteers
No
Inclusion criteria
* Age 65 years and older
Exclusion criteria
* known haematological condition (myelodysplasia, leukemia, cancer) * inability to provide written informed consent
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| Gene Set Enrichment Analysis (GSEA) Normalized Enrichment Score (NES) | One day |
Countries
Belgium
Contacts
CONTACTMichaël R. Laurent, MD PhD
PRINCIPAL_INVESTIGATORNick Van Gastel, PhD
de Duve Institute
Outcome results
None listed