Inflammatory Response, Oxidative Stress, Oxidative Stress Induction
Conditions
Brief summary
The aim of this research project is to explore the acute impact of specific foods and beverages, which have been shown to be associated with inflammatory processes, mainly in epidemiological studies, on inflammatory and oxidative stress parameters in healthy humans following a high intensity physical workout.
Detailed description
The aim of this research project is to explore the acute impact of specific foods and beverages, which have been shown to be associated with inflammatory processes, mainly in epidemiological studies, on inflammatory and oxidative stress parameters in healthy humans following a high intensity physical workout. The choice of food will mainly be based on the food groups from the Empirical Dietary Inflammatory Index. For that purpose, forty-two healthy volunteers will be recruited to perform several interventions in order to assess the impact of different nutrient groups or single- foods on oxidative and inflammatory markers. They will be divided into two groups (beverage consumption, n=20 & plantbased foods, n=22). The intense physical activity serves to induce a physiological stress response in the body, known to be accompanied by an increase in oxidative and inflammatory stress parameters. The idea is that anti- inflammatory or antioxidant nutrients could potentially attenuate this stress response in dependence on their anti- inflammatory or antioxidant potential. The operability of this concept has already been tested. The main benefit of this approach is to get a deeper insight into short term effects of single foods or beverages on inflammatory and prooxidative processes in humans by using short term intense physical activity as a stress model. One major practical aspect of this approach is to assess dietary components with respect to their pro- or anti-inflammatory-/oxidative stress potential regarding e.g. non-communicable diseases such as type 2 diabetes, coronary heart disease or the metabolic syndrome. Furthermore, it could help to reduce inflammation or oxidative stress during or following high intense physical exercise in order to reduce the stress response during training or competition in order to enhance regeneration and to foster the training gain in sports.
Interventions
OTHERNutrition
acute single dose nutrition intervention
Sponsors
University of Vienna
Study design
Allocation
RANDOMIZED
Intervention model
CROSSOVER
Primary purpose
PREVENTION
Masking
NONE
Intervention model description
acute consumption of anti- and pro- inflammatory foods (n=22) and beverages (n=20) 2 hour prior to intense physical activity in healthy female subjects (in total 42 subjects)
Eligibility
Sex/Gender
FEMALE
Age
18 Years to 40 Years
Healthy volunteers
Yes
Inclusion criteria
* female human subjects * aged 18-40 years * no chronical diseases * not more than 120 minutes of physical activity per week * no regular resistance training within the past 6 months * BMI 18.5 - 24.99 kg/m² * body fat percentage of at least 20% *
Exclusion criteria
* contraindications against physical activity * anemia * fructose or lactose intolerance * smoking * medications with influence on ROS levels or inflammation * allergy or intolerance against intervention food/beverages.
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| ROS change | change from baseline to 2.5 hours | ROS (change from baseline = after overnight fasting to post exercise (=after 2.5 hours) |
| IL-6 change | change from baseline to 2.5 hours | IL-6 (change from baseline = after overnight fasting to post exercise (=after 2.5 hours) |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| MDA change | change from baseline to 2.5 hours | MDA (change from baseline = after overnight fasting to post exercise (=after 2.5 hours) |
| hs-CRP change | change from baseline to 2.5 hours | hs-CRP (change from baseline = after overnight fasting to post exercise (=after 2.5 hours) |
| FRAP | change from baseline to 2.5 hours | Ferric Reducing Antioxidant Power Assay |
| estradiol | baseline | 17-beta-estradiol |
| Lipidomics | change from baseline to 2.5 hours | oxLDL |
| Protein Carbonyls | change from baseline to 2.5 hours | Protein Carbonyl Assay |
| Polyphenol Content | change from baseline to 2.5 hours | Polyphenol Content Plasma |
| DNA damage (comet assay | change from baseline to 2.5 hours | comet assay - wholeblood |
Countries
Austria
Outcome results
None listed