Resident Memory T Cells in Vitiligo

Tissue Resident Memory T Cells in Early & Late Cases of Generalized Non-segmental Vitiligo

Status

UNKNOWN

Phases

Unknown

Study type

Observational

Source

ClinicalTrials.gov

Registry ID

NCT05223738

Enrollment

Registered

2022-02-04

Start date

2020-12-31

Completion date

2022-02-01

Last updated

2022-02-04

For informational purposes only — not medical advice. Sourced from public registries and may not reflect the latest updates. Terms

Conditions

Vitiligo

Brief summary

This study aims to detect the presence and diversity of Tissue resident memory T cell populations in early and late cases of generalized non-segmental Vitiligo.

Detailed description

* Written informed consent obtained from every participant. * Detailed history taking including onset, course, duration of the disease, aggravating factors and received medications * VASI score assessed in every patient. * Patients photographed, divided into two groups early & late cases and 3 mm punch biopsies will be taken from perilesional and non lesional skin. * Biopsies processed and stained for CD8 & CD69 in cases of early group, & late group and controls by immunofluorescence.

Interventions

None listed

Study design

Observational model

CASE_CONTROL

Time perspective

PROSPECTIVE

Eligibility

Sex/Gender

ALL

Age

18 Years to 60 Years

Inclusion criteria

* \- Patients with Generalized non-segmental Vitiligo ( lesions in more than one anatomical site ). * Both genders. * Early group: onset less than or equal 6 months. * Late group: onset more than 6 months

Exclusion criteria

* Localized or Segmental Vitiligo. * Age \< 14 years. * Pregnant females * Patient who received treatment of any type within the last 3 months for the late group. * Patients with other skin diseases or immunosuppression

Design outcomes

Primary

Measure	Time frame	Description
CD8+ immunofluorescence	6months-1 year	Number of Tissue resident Memory cells by positively stained by CD8+ in early and late generalized non segmental vitiligo patients
CD69+ immunofluorescence	6months-1 year	Number of Tissue resident Memory cells by positively stained by CD8+ in early and late generalized non segmental vitiligo patients
Double CD8+ and CD69+ immunofluorescence	6months-1 year	Number of Tissue resident Memory cells by double stained by CD8+ &CD 69+ in early and late generalized non segmental vitiligo patients
VASI	6months-1 year	Vitiligo Area and Severity index minimum 0 maximum 100, the larger the number the worse the condition

Countries

Egypt

Outcome results