Quantitation of Bacteria Associated With Severe Periodontitis by Real -Time PCR Following Application of Diode Laser

Quantitave Assessment of Aggregatibacter Actinomycetemcomitans and Porphyromonas Gingivalis by Real -Time PCR Following Application of Diode Laser in Severe Periodontitis (A RANDIOMIZED CONTROLLED CLINICAL TRIAL)

Status

Completed

Phases

Phase 2Phase 3

Study type

Interventional

Source

ClinicalTrials.gov

Registry ID

NCT05222737

Enrollment

Registered

2022-02-03

Start date

2021-01-01

Completion date

2021-12-01

Last updated

2022-07-06

For informational purposes only — not medical advice. Sourced from public registries and may not reflect the latest updates. Terms

Conditions

Periodontal Pocket

Brief summary

1. Primary objective To measure the gingival crevicular levels of A.actinomycetemcomitans and P.gingivalis after intra-pocket application of diode laser in severe periodontitis (stage 3 grade C) patients. 2. Secondary objective To compare the levels of the same microorganisms as in cases of diode laser therapy to those after conventional treatment with systemic antibiotic administration in severe periodontitis (stage 3 grade C) patients. 3. to measure clinical parameters probing depth,attachment loss and mobility grade

Interventions

DEVICEmedency diode laser

intra pocket application of diode laser

DRUGspirazole forte

adminstration of spirazole forte with a dose 17 mg per kg

Study design

Allocation

RANDOMIZED

Intervention model

PARALLEL

Primary purpose

TREATMENT

Masking

DOUBLE (Subject, Caregiver)

Eligibility

Sex/Gender

ALL

Age

15 Years to 35 Years

Healthy volunteers

Yes

Inclusion criteria

1. Patients of both sexes having severe periodontitis (stage 3 grade C) with interdental clinical attachment loss (CAL) ≥ 5. 2. Patients' age between 15 and 35 years old with rapid bone loss showed by radiograph. 3. Systemically healthy patients. 4. Radiographic bone loss extending to middle or apical third of root. 5. Probing depth (PD) ≥ 6 mm.

Exclusion criteria

1. Use of antibiotics during the last 3 months. 2. Pregnancy. 3. Any systemic condition that might affect the study. 4. Use of mouthwash containing antimicrobials during the previous 3 weeks. 5. Smoking.

Design outcomes

Primary

Measure	Time frame	Description
Quantitation of Aggrecatibacter .actinomycetemcomitans and porphyromonas gingivalis bacteria	baseline	To measure the gingival crevicular levels of A.actinomycetemcomitans and P.gingivalis after intra-pocket application of diode laser in severe periodontitis(stage 3 grade C) patients

Secondary

Measure	Time frame	Description
comparing levels of bacteria between test and control groups	baseline	To compare the levels of the same microorganisms as in cases of diode laser therapy to those after conventional treatment with systemic antibiotic administration in severe periodontitis ( stage 3 grade C) patients.

Other

Measure	Time frame	Description
Probing depth(PPD)	Baseline ,one month and 3 months	Measure PPD at baseline and after treatment
Clinical Attachment loss(CAL)	Baseline ,one month and 3 months	Measure CAL at baseline and after treatment
Mobility grade	Baseline ,one month and 3 months	Measure mobility grade at baseline and after treatment

Countries

Egypt

Outcome results

None listed

Source: ClinicalTrials.gov · Data processed: Feb 7, 2026