Periodontitis, Systemic Sclerosis
Conditions
Keywords
systemic sclerosis, periodontitis, antioxidants, saliva
Brief summary
The aim of the research will be to evaluate the effects of NSPT on clinical periodontal parameters, level of antioxidants in unstimulated saliva and the levels of biomarkers (VEGF, TGF-β, IL-17) in the GCF in SSc patients in comparison with systemically healthy controls. Twenty systemic sclerosis patients with periodontitis (SS group) and twenty systemically healthy periodontitis patients (P group) will be enrolled in the present study. The results may indicate higher periodontal destruction and antioxidant perturbations in unstimulated saliva of systemic sclerosis patients with periodontitis compared to systemically healthy periodontitis patients.
Interventions
PROCEDUREperiodontal examination's, determination of salivary antioxidants and cytokine levels (VEGF, TGF-β, Il-17) in gingival crevicular fluid, Non-surgical therapy of periodontitis
Periodontal parameters has been recorded on six sites of each tooth using a manual periodontal probe. Activity of antioxidants in unstimulated saliva (GPX, SOD, UA) has been determined using the colorimetric method and commercial kits. Total cytokine levels (VEGF, TGF-β, Il-17) in gingival crevicular fluid were determined using commercial ELISA (enzyme-linked immunosorbent assay) kits . The therapeutic procedure begun with ultrasonic scaling (MiniPiezon, EMS Electro Medical Systems, Switzerland), along with education and motivation of subjects to maintain oral hygiene properly. Periodontal debridement was carried out under local anesthesia, by quadrants, starting from the upper right quadrant at 7-day intervals until the completion of therapy. Instrumentation of all periodontal pockets was processed manually using specialized curettes.
DIAGNOSTIC_TESTActivity of antioxidants in unstimulated saliva (GPX, SOD, UA)
Activity of antioxidants in unstimulated saliva (GPX, SOD, UA) has been determined using the colorimetric method and commercial kits.
DIAGNOSTIC_TESTTotal cytokine levels (VEGF, TGF-β, Il-17) in gingival crevicular fluid
Total cytokine levels (VEGF, TGF-β, Il-17) in gingival crevicular fluid were determined using commercial ELISA (enzyme-linked immunosorbent assay) kits .
PROCEDUREPeriodontal treatment
The therapeutic procedure begun with ultrasonic scaling (MiniPiezon, EMS Electro Medical Systems, Switzerland), along with education and motivation of subjects to maintain oral hygiene properly. Periodontal debridement was carried out under local anesthesia, by quadrants, starting from the upper right quadrant at 7-day intervals until the completion of therapy. Instrumentation of all periodontal pockets was processed manually using specialized curettes.
Sponsors
University of Belgrade
Study design
Allocation
NON_RANDOMIZED
Intervention model
PARALLEL
Primary purpose
TREATMENT
Masking
NONE
Eligibility
Sex/Gender
ALL
Healthy volunteers
No
Inclusion criteria
* established diagnoses of systemic sclerosis and periodontitis (study group), * age ≥ 18 * presence of minimum 12 teeth
Exclusion criteria
* concurrent inflammatory mediated rheumatic diseases * pregnancy or lactation * active cigarette smokers * a history of periodontal, antibiotics or non-steroidal drug therapy in the past 6 months.
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| plaque index (PI) | one day | Evaluation of oral hygiene status was recorded with a Silness Loe plaque index (PI). |
| Clinical attachment level (CAL) | one day | Measuring the CAL by periodontal probe, measured in mm |
| Probing depth (PD) | one day | Measuring the PD by periodontal probe, measured in mm |
| Gingival margin level (GML) | one day | Measuring the GML by periodontal probe, measured in mm |
| The degree of gingival inflammation | one day | The degree of gingival inflammation was assessed using the Loe and Silness (LSGI) gingival index. |
| The concentration of vascular endothelial growth factor (VEGF) | baseline and two months after periodontal treatment | The level of VEGF were determined using commercial ELISA (enzyme-linked immunosorbent assay) kits. |
| The concentration of transforming growth factor beta (TGF-β) | baseline and two months after periodontal treatment | The level of TGF-β in gingival crevicular fluid was determined using commercial ELISA (enzyme-linked immunosorbent assay) kits at the Implant Research Center, School of Dental Medicine, University of Belgrade, following the manufacturer's instructions. |
| The concentration of interleukin (IL)-17 | baseline and two months after periodontal treatment | interleukin (IL)-17 was determined using commercial ELISA (enzyme-linked immunosorbent assay) kits at the Implant Research Center, School of Dental Medicine, University of Belgrade, following the manufacturer's instructions. |
| The concentration of glutathion peroxidase (GPX) | one day | Activity level of GPX, determined using the colorimetric method and commercial kits. |
| The concentration of uric acid (UA) | one day | Activity level of UA, determined using the colorimetric method and commercial kits. |
| The concentration of superoxide dismutase (SOD) | one day | Activity level of SOD, determined using the colorimetric method and commercial kits. |
| The concentration of tumor necrosis factor alpha | baseline and two months after periodontal treatment | — |
| The concentration of C reactive protein | baseline and two months after periodontal treatment | — |
| Erythrocyte sedimentation rate (ESR) | baseline and two months after periodontal treatment | — |
Countries
Serbia
Contacts
Primary ContactNatasa Nikolic Jakoba, DDS, PhD
Outcome results
None listed