Postexercise Hypotension
Conditions
Keywords
Histamine, Mast cell, Exercise
Brief summary
This study is investigating the role of histamine in generating adaptation to exercise
Detailed description
Exercise promotes and maintains healthy cardiovascular, musculoskeletal, and metabolic function, but the signals and mechanisms which transduce these effects are poorly understood. Histamine plays a role in some of the positive benefits of exercise. The goal of this study is to determine the factors that regulate exercise's effects on endothelial and vascular function, with a focus on histamine released from mast cells in skeletal muscle. Participants will perform exercise or participate in interventions like heating that may replicate some of the effects of exercise. During most experiments, investigators will insert an intravenous catheter in an arm vein and microdialysis probes in the leg, collect dialysate from the microdialysis probe and blood from the vein, record noninvasive measures, and have the participants perform exercise or undergo heating.
Interventions
BEHAVIORALAerobic Exercise
Subjects will complete a single bout of aerobic exercise.
DRUGalpha-FMH
Subjects will undergo either local heating with diathermy or whole body heating with far-infrared sauna.
BEHAVIORALResistance and Aerobic Exercise
Subjects will complete bouts of resistance and aerobic exercise.
DRUGAntihistamine
Subjects will complete a single bout of aerobic exercise under placebo vs antihistamine conditions.
Sponsors
University of Oregon
Study design
Allocation
NON_RANDOMIZED
Intervention model
PARALLEL
Primary purpose
BASIC_SCIENCE
Masking
TRIPLE (Subject, Investigator, Outcomes Assessor)
Intervention model description
This is a series of Basic Experimental Studies with Humans (BESH, as defined by PA-20-184). Subjects are enrolled (non-randomized) in different arms of the overall study.
Eligibility
Sex/Gender
ALL
Age
18 Years to 40 Years
Healthy volunteers
Yes
Inclusion criteria
* Age 18 -40
Exclusion criteria
* Systolic ≥ 120 * Diastolic ≥ 80 * Body mass index (BMI) ˃ 28 kg/m2 * Prior diagnosis of cardiovascular disease, diabetes, autonomic disorders, or asthma * Smoking or nicotine use * Ongoing medical therapy (other than birth control) * Ongoing use of over-the-counter or prescription antihistamines * Allergies or hypersensitivities to drugs, local anesthetics, skin disinfectants, adhesives, or latex * Pregnant, breastfeeding subjects, or planning to become pregnant in the next 12 months * Mobility restrictions that interfere with physical activity * High physical activity based on International Physical Activity Questionaire (IPAQ1) * Non-English speaking
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Percentage of mast cell degranulation | One hour | Beta-hexosaminidase release from mast cells in mast cell degranulation bioassay when exposed to intramuscular dialysate from exercising individuals |
| Histamine concentration | One hour | Histamine concentration in intramuscular dialysate |
| Histamine metabolites | 24 hours | Concentration of histamine metabolites in blood and urine |
| Muscle perfusion | One hour | Skeletal muscle tissue oxygen index |
Countries
United States
Contacts
PRINCIPAL_INVESTIGATORJohn R Halliwill, PhD
University of Oregon
Outcome results
None listed