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Role of the Protein Matrix in the Anabolic Response to a Ground Beef Patty as Opposed to the Impossible (Vegi-) Burger

Role of the Protein Matrix in the Anabolic Response to a Ground Beef Patty as Opposed to the Impossible (Vegi-) Burger

Status
Completed
Phases
NA
Study type
Interventional
Source
ClinicalTrials.gov
Registry ID
NCT05197140
Enrollment
33
Registered
2022-01-19
Start date
2022-05-01
Completion date
2022-11-03
Last updated
2024-08-05

For informational purposes only — not medical advice. Sourced from public registries and may not reflect the latest updates. Terms

Conditions

Protein Metabolism

Brief summary

Several of the investigator's recent studies have shown that all dietary proteins are not equal when it comes to making proteins in human bodies. Generally, foods made from animal muscle tissue are much more efficient at making new proteins in the body that those from plant sources. This study will measure the amount of protein growth in participants after consuming either one beef patty, one vegi-burger, or two vegi-burgers. By using stable isotope infusions and collecting blood and leg muscle samples, the investigator's lab can determine the rate of muscle metabolism that happens after a participant eats a protein-containing food. The investigator wants to determine if the vegi-burger is as efficient as the beef patty at creating proteins in a participants. The investigator will measure this metabolism over a 10-hour period, with the food being eaten at the 4 hour mark. The investigator plans to perform this procedure on up to 8 participants per food option (24 total).

Detailed description

According to the USDA Economic Research Service Food Availability Data, beef consumption has been on a general decline for the past 40 years, with current per capita consumption approximately 35% lower in 2020 than in 1980. Some of this decline over the past few years can be attributed to politically motivated claims that carbon emissions from cattle represent a major threat to the global environment. However, the major reason for declining beef consumption is the widely promulgated claim that beef is unhealthy. The hamburger in particular has become a poster child for the notion that beef consumption is responsible for the increasing occurrence of obesity in the United States. The trend of decreasing beef consumption due to perceived health concerns, particularly obesity and associated health problems, has led major fast-food chains such as Burger King and McDonalds, to offer vegetarian burgers as healthy alternatives to the traditional beef burger. However, examination of the components of the macronutrient matrix of a typical vegetarian burger gives reason to question the health benefits as compared to a traditional beef patty-hamburger.

Interventions

OTHERfood: cooked 85% lean ground beef

see arm description

OTHERfood: Impossible Burger (R)

see arm description

Sponsors

University of Arkansas
Lead SponsorOTHER

Study design

Allocation
RANDOMIZED
Intervention model
PARALLEL
Primary purpose
BASIC_SCIENCE
Masking
SINGLE (Investigator)

Masking description

Investigator will be blinded to group.

Eligibility

Sex/Gender
ALL
Age
18 Years to 40 Years
Healthy volunteers
Yes

Inclusion criteria

* Ages 18 - 40 yrs. * Body Mass Index of 20-32 inclusive

Exclusion criteria

* Unwilling to eat animal proteins * History of diabetes that requires medication for control of blood glucose * History of malignancy or chemo/radiation therapy in the 6 months prior to enrollment * History of gastrointestinal bypass/reduction surgery (Lapband, gastric sleeve, etc.) * Pregnant females * Hemoglobin less than 12 g/dL at screening * Platelets less than 150,000 at screening * Subjects who cannot refrain from using protein or amino acid supplements for 7 days prior to Visit 3 * Concomitant use of oral or injectable corticosteroids * Concomitant use of testosterone, IGF-1, or similar anabolic agent * Any other disease or condition that would place the subject at increased risk of harm if they were to participate, at the discretion of the study physician

Design outcomes

Primary

MeasureTime frameDescription
Postprandial Plasma Essential Amino Acid Concentration Incremental Area-under-the-curve [ Time Frame: 360 Minutes]6 hours after consumption of interventionFree leucine, isoleucine, valine, histidine, lysine, methionine, phenylalanine, threonine, tryptophan (combined) (µmol·L\^(-1)·min). Following time points (in minutes) post-ingestion will be used to calculate the incremental area under the curve: 60, 90, 120, 180, 210, 240, 270, 300, 330, 360.

Countries

United States

Participant flow

Participants by arm

ArmCount
4 Ounce Beef Patty
Subjects consume one beef patty of 4 ounces of cooked meat. food: cooked 85% lean ground beef: see arm description
8
4 Ounce Vegetarian Burger
Subjects consume one patty of 4 ounces of cooked vegetarian product. food: Impossible Burger (R): see arm description
8
2x 4 Ounce Vegetarian Burger
Subjects consume two patties of 4 ounces of cooked vegetarian product. food: Impossible Burger (R): see arm description
8
Total24

Baseline characteristics

Characteristic4 Ounce Vegetarian Burger2x 4 Ounce Vegetarian BurgerTotal4 Ounce Beef Patty
Age, Categorical
<=18 years
0 Participants0 Participants0 Participants0 Participants
Age, Categorical
>=65 years
0 Participants0 Participants0 Participants0 Participants
Age, Categorical
Between 18 and 65 years
8 Participants8 Participants24 Participants8 Participants
Age, Continuous36.5 years
STANDARD_DEVIATION 4.2
28.9 years
STANDARD_DEVIATION 5.8
32.5 years
STANDARD_DEVIATION 6.5
30.0 years
STANDARD_DEVIATION 7.4
Race (NIH/OMB)
American Indian or Alaska Native
0 Participants0 Participants0 Participants0 Participants
Race (NIH/OMB)
Asian
0 Participants0 Participants0 Participants0 Participants
Race (NIH/OMB)
Black or African American
0 Participants1 Participants2 Participants1 Participants
Race (NIH/OMB)
More than one race
0 Participants0 Participants0 Participants0 Participants
Race (NIH/OMB)
Native Hawaiian or Other Pacific Islander
0 Participants0 Participants0 Participants0 Participants
Race (NIH/OMB)
Unknown or Not Reported
0 Participants0 Participants0 Participants0 Participants
Race (NIH/OMB)
White
8 Participants7 Participants22 Participants7 Participants
Region of Enrollment
United States
8 participants8 participants24 participants8 participants
Sex: Female, Male
Female
5 Participants5 Participants14 Participants4 Participants
Sex: Female, Male
Male
3 Participants3 Participants10 Participants4 Participants

Adverse events

Event typeEG000
affected / at risk
EG001
affected / at risk
EG002
affected / at risk
deaths
Total, all-cause mortality
0 / 80 / 80 / 8
other
Total, other adverse events
0 / 80 / 80 / 8
serious
Total, serious adverse events
0 / 80 / 80 / 8

Outcome results

Primary

Postprandial Plasma Essential Amino Acid Concentration Incremental Area-under-the-curve [ Time Frame: 360 Minutes]

Free leucine, isoleucine, valine, histidine, lysine, methionine, phenylalanine, threonine, tryptophan (combined) (µmol·L\^(-1)·min). Following time points (in minutes) post-ingestion will be used to calculate the incremental area under the curve: 60, 90, 120, 180, 210, 240, 270, 300, 330, 360.

Time frame: 6 hours after consumption of intervention

ArmMeasureValue (MEAN)Dispersion
4 Ounce Beef PattyPostprandial Plasma Essential Amino Acid Concentration Incremental Area-under-the-curve [ Time Frame: 360 Minutes]117896 micromoles per liter per minuteStandard Deviation 45787
4 Ounce Vegetarian BurgerPostprandial Plasma Essential Amino Acid Concentration Incremental Area-under-the-curve [ Time Frame: 360 Minutes]75098 micromoles per liter per minuteStandard Deviation 45231
2x 4 Ounce Vegetarian BurgerPostprandial Plasma Essential Amino Acid Concentration Incremental Area-under-the-curve [ Time Frame: 360 Minutes]94058 micromoles per liter per minuteStandard Deviation 27252

Source: ClinicalTrials.gov · Data processed: Feb 6, 2026