Anesthesia, DNA Damage
Conditions
Keywords
Desflurane, Intravenous anesthetics, propofol, comet essay
Brief summary
The purpose of this study is to evaluate if the anesthetics propofol and desflurane can damage DNA according to comet essay in patients submitted to lumbar disc surgery.
Detailed description
It is expected that DNA damage information provide the possible genotoxic effects of inhalational and intravenous anesthetics and contribute to the establishment of strategies aimed at reducing the risks to exposed patients.
Interventions
DRUGAnesthetics, General Desflurane Maintenance
Comparison of dna damage of desflurane maintenance with propofol maintenance according to comet analysis
DRUGAnesthetics, General Propofol Maintenance
Comparison of dna damage of propofol maintenance with desflurane maintenance according to comet analysis
Sponsors
Muğla Sıtkı Koçman University
Study design
Allocation
RANDOMIZED
Intervention model
SEQUENTIAL
Primary purpose
DIAGNOSTIC
Masking
DOUBLE (Subject, Outcomes Assessor)
Eligibility
Sex/Gender
ALL
Age
18 Years to 65 Years
Healthy volunteers
Yes
Inclusion criteria
1. Ability to understand and willingness to sign an informed consent form 2. Elective lumbar spinal surgery that will take at least 120 minutes, 3. American Society of Anesthesiologists (ASA) physical status I and II
Exclusion criteria
1. Patients with ASA physical status III or IV disease 2. Chronic systemic disease (diabetes mellitus,chronic pulmonary disorders, chronic kidney and liver disorders) 3. Smoking or alcohol consumption 4. Recently received radiation, chemotherapy 5. Malignancy 6. Patients who will receive blood transfusion during the operation 7. Users of antioxidant supplements 8. Patients who have received general anesthesia in the last three months 9. Patients with known occupational exposures (operating room personnel, chemical plant worker
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Intensity of the DNA tail detected by using Comet Assay IV Version 4.3.2 for Basler FireWire | Before anesthesia induction | The blood samples that evaluate with comet analysis aim to detect the intensity of the DNA tail, and the tail greater, the greater the damage. |
| Head length of DNA detected by using Comet Assay IV Version 4.3.2 for Basler FireWire | Before anesthesia induction | The blood samples that evaluate with comet analysis aim to detect the head length of DNA, the head greater, the smaller the damage. |
| Tail length of DNA detected by using Comet Assay IV Version 4.3.2 for Basler FireWire | before anesthesia induction | The blood samples that evaluate with comet analysis aim to detect the tail length of DNA, the head greater, the greater the damage. |
Countries
Turkey (Türkiye)
Outcome results
None listed