Orthopedic Surgery
Conditions
Keywords
biofilm antibiotics
Brief summary
Detection of bacteria that form biofilm and its sensitivity to antibiotics in patients with surgical site infection after orthopedic surgery.
Detailed description
The available number of patients presenting in outpatient clinic or at operating theatre with surgical site infection after orthopedic surgery that include insertion of implant (e.g plates , screws ,wires ,…) will be subjected to full history and examined by physician. Swabs from site of infection will be taken and subjected to the following steps to identify the causative bacteria: * Culturing on blood agar, chocolate agar, MacConkey agar and Mannitol salt agar , gram staining and biochemical tests. * Enzyme-linked immunosorbent assay (ELISA) to detect biofilm formation. * Antimicrobial Susceptibility Testing using the Kirby-Bauer disk diffusion method.
Interventions
DIAGNOSTIC_TESTswab
Swabs from site of infection will be taken
Sponsors
Sohag University
Study design
Observational model
OTHER
Time perspective
CROSS_SECTIONAL
Eligibility
Sex/Gender
ALL
Healthy volunteers
No
Inclusion criteria
* patients with surgical site infection after orthopedic surgery that include insertion of implant (e.g plates , screws ,wires ,…)
Exclusion criteria
* if patient has no orthopedic surgery
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| detect biofilm formation | one year | The available number of patients presenting in outpatient clinic or at operating theatre with surgical site infection after orthopedic surgery that include insertion of implant (e.g plates , screws ,wires ,…) will be subjected to full history and examined by physician. Swabs from site of infection will be taken and subjected to Enzyme-linked immunosorbent assay (ELISA) to detect biofilm formation. |
Contacts
Primary Contactprincipal investigator
Outcome results
None listed