Exercise, Histamine
Conditions
Brief summary
Blocking histamine H1/H2 receptors blunts chronic endurance training adaptations. The current study addresses a twofold research question: What is the influence of endurance training (1) and histamine H1 and H2 signaling (2) on the gene expression in human skeletal muscle. Results from this study will yield more insights into the molecular mechanisms of adaptations to exercise training.
Interventions
DRUGPlacebo: Placebo + exercise training.
Oral placebo Exercise training: Interval-based cycling exercise
DRUGH1 blockade: H1 receptor antagonist + exercise training
H1 blockade: oral blockade Exercise training: Interval-based cycling exercise
DRUGH2 Blockade: H2 receptor antagonist + exercise training
H2 blockade: oral blockade Exercise training: Interval-based cycling exercise
Sponsors
Research Foundation Flanders
University Ghent
Study design
Allocation
RANDOMIZED
Intervention model
CROSSOVER
Primary purpose
BASIC_SCIENCE
Masking
TRIPLE (Subject, Investigator, Outcomes Assessor)
Intervention model description
Randomized double-blinded placebo-controlled cross-over interventional study
Eligibility
Sex/Gender
ALL
Age
18 Years to 45 Years
Healthy volunteers
Yes
Inclusion criteria
* Male and female * 18-45 years * not to medium physically active
Exclusion criteria
* Smoking * Chronic disease * Supplement or medication intake * Seasonal allergies
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Change in muscle transcriptome (direct response) | On each test-day: 0 minutes after the exercise training versus at rest. | RNA-sequencing |
| Change in muscle transcriptome (delayed response) | On each test-day: 180 minutes after the exercise training versus at rest. | RNA-sequencing |
| Muscle glycogen depletion | On each test-day: 0 minutes after the exercise training versus at rest. | Fluorometric determination of muscle glycogen levels |
| Muscle glycogen resynthesis | On each test-day: 180 minutes after the exercise training versus at rest. | Fluorometric determination of muscle glycogen levels |
| Plasma volume change | Change from rest to different time-points after exercise (0, 30, 60, 120 and 180 minutes after the exercise training). | Plasma volume based on hemoglobin and hematocrit concentration. |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| Heart rate during the exercise training | Continuously during training on each test-day. | Heart rate during the exercise training. |
| Muscle signaling pathways relevant for glucose metabolism and cardiovascular health | On each test-day: at rest, 0 minutes and 180 minutes after the exercise training. | Phosphorylation status of proteins assessed by Western Blotting |
| Blood lactate | On each test-day at 10 time-points: at rest, during exercise and 0, 30, 60, 120 and 180 minutes after the exercise training. | Capillary lactate concentration. |
| Blood glucose | On each test-day at 10 time-points: at rest, during exercise and 0, 30, 60, 120 and 180 minutes after the exercise training. | Capillary glucose concentration. |
| Blood histamine | On each test-day at 6 time-points: at rest and 0, 30, 60, 120 and 180 minutes after the exercise training. | histamine concentration in blood samples. |
| Blood insulin | On each test-day at 6 time-points: at rest and 0, 30, 60, 120 and 180 minutes after the exercise training. | insulin concentration in blood samples. |
Countries
Belgium
Outcome results
None listed