Oxidative Stress
Conditions
Keywords
antioxidant vitamins, oxidative damage
Brief summary
Determine if acute supplementation with antioxidant vitamins (vitamin C and vitamin E) influences the muscle damage and fatigue caused after an acute session of eccentric training.
Detailed description
A randomized, single-blind, placebo-control parallel trial will be conducted. In a previous session volunteers will be interviewed to determine if they meet the inclusion criteria. Participants should avoid ingesting of foods that contain high amounts of antioxidant compounds: more than two juices of fruits, more than four cups of coffee or tea. Juices with high antioxidant content should be avoided. Alcoholic beverages should also be avoided. Participants will be divided in to groups and will performance one resistance exercise sessions. Prior to exercise, one group will intake supplementation VitC (1000 mg) + VitE (235 mg) and the other supplementation with placebo. The following sequence will be performed in each session: 1) baseline assessment (analysis of the composition body, blood draw and parameters of performance) prior to exercise session ; 2) administration of vitamin or placebo supplementation 2 hours before exercise session ; 3) post-exercise assessments (blood biomarkers and performance parameters) immediately after the session and after 24 hours. DOMS will also be assessed 48 h and 72 h after training. The training session implemented will be designed by a Sport Scientific
Interventions
DIETARY_SUPPLEMENTDietary Supplement: Vitamin C and vitamin E
Antioxidant supplementation
DIETARY_SUPPLEMENTPlacebo
Placebo administration
Sponsors
Universidad Europea de Madrid
Study design
Allocation
RANDOMIZED
Intervention model
PARALLEL
Primary purpose
TREATMENT
Masking
QUADRUPLE (Subject, Caregiver, Investigator, Outcomes Assessor)
Eligibility
Sex/Gender
ALL
Age
18 Years to 33 Years
Healthy volunteers
Yes
Inclusion criteria
* Healthy * Physically active
Exclusion criteria
* Injuries * Supplementation or drugs * Smokers
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Percentage of body fat and muscle mass | Prior to the intervention | Determination of body composition using skinfolds measurements |
| DOMS | 0 hours post exercise | Delayed onset muscle soreness. This outcome will be measure with a 10-point visual analogic scale (0= no pain; 10 = worst possible pain) |
| Blood malondialdehyde (MDA) | 2 hours prior to exercise | MDA will be analyzed from a blood sample |
| Blood creatine quinase (CK) | 2 hours prior to exercise | CK will be analyzed from a blood sample |
| Jump height in a Counter Movement Jump | 2 hours prior to exercise | Jump height (cm) in a counter movement jump measured with Octojump Platform |
| Jump Height in a Squat Jump | 24 h after exercise | Jump Height (cm) in a Squat Jump measured with Octojump Platform |
| Jump Height in a squat Jump | 2 hours prior to exercise | Jump height (cm) in a squat jump with Octojump Platform measured with Octojump Platform |
| Jump Height in a Drop Jump | 2 hours prior to exercise | Jump height (cm) was measured in different drop jumps with Octojump Platform |
| Contact time in a Drop Jump | Inmediately after exercise | Contact time (s)measured in different drop jumps with Octojump Platform |
| Concentration of blood lactate | 2 hours before exercise | Capillary blood lactate with Lactate Pro 2 |
Countries
Spain
Outcome results
None listed