DNA Damage
Conditions
Keywords
Desflurane,, DNA methylation, epigenetics, inhalation anesthetics
Brief summary
The purpose of this study is to demonstrate the molecular effect of desflurane on DNA by measuring the global DNA methylation level in patients under going lumbar spinal stenosis surgery under general anesthesia.
Detailed description
Forty patients scheduled for lumbar spinal surgery will include in the study. Peripheral venous blood samples will collect prior to the induction of anesthesia (T1) and after 120 minutes (T2). Anesthesia will maintaine with 40%-60% O2-air mixture and 6% desflurane. DNA will obtain from blood samples with the help of DNA isolation kit and global DNA methylation analysis will perform by ELISA method.
Interventions
DRUGDesflurane
Peripheral venous blood samplesof patients will collected at two time points as follows: before the premedication and the induction of anesthesia (T₁): 120 minutes after the beginning of anesthesia (T₂). Anesthesia maintenance will be provided with 40% O₂ and minimum alveolar concentration (MAC) in 60% air with 6% desflurane. DNA will be obtained from blood samples with the help of DNA isolation kit, and global DNA methylation analysis will performed by ELISA method.
Sponsors
Muğla Sıtkı Koçman University
Study design
Observational model
OTHER
Time perspective
PROSPECTIVE
Eligibility
Sex/Gender
ALL
Age
18 Years to 65 Years
Healthy volunteers
No
Inclusion criteria
1. Ability to understand and willingness to sign an informed consent form 2. Elective lumbar spinal surgery that will take at least 120 minutes, 3. American Society of Anesthesiologists (ASA) physical status I and II 4. Be between the ages of 18 and 65
Exclusion criteria
1. Patients with ASA physical status III or IV disease 2. Smoking or alcohol consumption 3. Chronic systemic disease (diabetes mellitus,chronic pulmonary disorders, chronic kidney and liver disorders 4. recently received radiation, chemotherapy 5. malignancy 6. blood transfusion in operation 7. use of antioxidant supplements 8. have received general anesthesia in the last three months 9. Patients with known occupational exposures (operating room personnel, chemical plant workers
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Global DNA methylation change 5 minutes before and 120 minutes after anesthesia induction in peripheral blood mononuclear cells | 5 minutes before induction of anesthesia ,120 minutes after the beginning of anesthesia | The global DNA methylation level will be measured 5 minutes before and 120 minutes after anesthesia induction. |
Countries
Turkey (Türkiye)
Outcome results
None listed