Child, Only, Essential Hypertension
Conditions
Keywords
cerebral circulation, child, endothelium, microcirculation, macrocirculation, hypertension, oxidative stress
Brief summary
The main goal of this study is to investigate the association of the juvenile essential arterial hypertension with systemic micro- and macrovascular reactivity and cerebral vascular function, and to examine the potential impact of elevated oxidative stress on this associations.
Interventions
None listed
Sponsors
Josip Juraj Strossmayer University of Osijek
Study design
Observational model
CASE_CONTROL
Time perspective
CROSS_SECTIONAL
Eligibility
Sex/Gender
ALL
Age
9 Years to 18 Years
Healthy volunteers
Yes
Inclusion criteria
* healthy normotensive children and children with essential arterial hypertension
Exclusion criteria
* secondary arterial hypertension * other systemic diseases with immunopathology * neurodegenerative diseasesat * children that use therapy which has a high impact on vascular or immunological function (immunotherapy, immunosuppressive therapy, sistemic corticosteroid therapy etc..).
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Systemic microvascular function in children | Day 1. | Skin microvascular reactivity assessed by Laser Doppler flowmetry (post-occlusive reactive hyperemia, iontophoresis of acetylcholine and sodium nitroprusside) - measured in perfusion units (PU) |
| Systemic macrovascular function in children | Day 1. | Vascular ultrasound measurement of brachial artery response to vascular occlusion (flow mediated dilation; FMD, %) |
| Cerabral vascular function in children | Day 1. | Cerebral blood flow velocities assessed by transcranial Doppler sonography (cm/s) |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| Oxidative stress - thiobarbituric acid reactive substances (TBARS) | Day 1. | Serum sample collection for measurement of biomarkers of oxidative stress level. Thiobarbituric acid reactive substances (TBARS) - biomarker of lipid peroxidation. |
| Endocan | Day 1. | Serum sample collection for ELISA measurement of serum endocan level - proteoglycan associated with endothelium activation |
Other
| Measure | Time frame | Description |
|---|---|---|
| Blood pressure | Day 1. | Automatic oscillometric measurement of blood pressure (in mmHg) |
| Body fluid status | Day 1. | Body fluid status measurement by a four-terminal portable impedance analyzer. Based on the value of skin resistance (Ohm) empirically derived formulas (the original manufacturer's software) will be used to calculate the estimated Total Body Water (TBW L), Extracellular Water (ECW L), Intracellular Water (ICW L), Plasma Fluid (PF L) and Interstitial Fluid (IF L). |
| Heart rate | Day 1. | Automatic oscillometric measurement of heart rate (bpm) |
| Body mass index | Day 1. | Measurement of body weight (kg) and body height (cm) from which will be combined to report body mass index (BMI) using formula: weight (kg) / \[height (m)\]2 (kg/m2) |
| Body composition - Fat Free Mass estimation | Day 1. | Body composition status measured by a fourterminal portable impedance analyzer. Based on the value of skin resistance (Ohm) empirically dervide formulas (the original manufacturer's software) will be used to calculate the estimated Fat Free Mass (FFM kg) |
| Body composition - Fat estimation | Day 1. | Body composition status measured by a fourterminal portable impedance analyzer. Based on the value of skin resistance (Ohm) empirically dervide formulas (the original manufacturer's software) will be used to calculate the estimated Fat (Fat Mass kg) |
Countries
Croatia
Contacts
Primary ContactMartina Kos, MD
Outcome results
None listed