COVID19, Immune Response, Vaccination
Conditions
Brief summary
Aim of this study is to evaluate whether COVID-19 vaccination induces a persistent cellular immune response. To this aim, blood samples are taken from vaccinated individuals and not immunized subjects as a control group. Cells isolated from blood samples are tested in vitro to assess the percentage of spike-specific T and B lymphocytes 1 and 7 months after a second dose of Comirnaty vaccine.
Detailed description
After providing written informed consent, 28 vaccinated and 25 non vaccinated subjects were included in the study. All subjects were enrolled among healthcare workers in the Research Centre of IRCCS San Raffaele Roma in Rome. All the subjects declared that they never tested positive for COVID19. Blood and serum samples were drowned twice from vaccinated subjects: 1 and 7 months after a second dose of vaccine to evaluate T and B response to vaccine; blood was drowned once in not vaccinated subjects. Cells were collected form peripheral blood samples, while anti-S1 IgG titre was assessed in serum. Flow-cytometry was used to assess the percentage of circulating spike-specific T and B lymphocytes.
Interventions
OTHERanalysis of immunological response
collection and analysis of SARS-CoV2 Spike specific blood cells
DIAGNOSTIC_TESTanalysis of serum antibodies
analysis of anti-SARS-CoV2 Spike specific antibodies
Sponsors
IRCCS San Raffaele Roma
Study design
Observational model
COHORT
Time perspective
PROSPECTIVE
Eligibility
Sex/Gender
ALL
Age
18 Years to No maximum
Healthy volunteers
Yes
Inclusion criteria
* vaccinated and non vaccinated subjects * subjects that never tested positive for COVID19
Exclusion criteria
* subjects that tested positive for COVID-19
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| measurement of cellular response | 7 months | evaluation of the increase of Spike-specific B and T cells percentage after COVID-19 vaccination |
Countries
Italy
Outcome results
None listed