Bacterial Infections
Conditions
Brief summary
This study aims to evaluate the effectiveness of ultrasonic irrigation in improving root canal disinfection after chemomechanical procedures.
Detailed description
Although chemomechanical procedures promote a drastic bacterial reduction, many root canals remain infected, which points to the need for complementary procedures to improve root canal disinfection. Thus, this clinical study aims to evaluate the effect of passive ultrasonic irrigation as a supplementary disinfection procedure after root canal preparation. Microbiological samples of the root canals of 30 single-rooted teeth with apical periodontitis will be taken at different stages of the endodontic treatment: before preparation (S1), after chemomechanical procedures (S2) and after passive ultrasonic irrigation (S3). The samples will be submitted to the quantitative polymerase chain reaction for bacterial quantification and to the reverse transcription quantitative polymerase chain reaction for bacterial activity analysis. Data will be analyzed using the Wilcoxon test for paired samples (p \< 0.05).
Interventions
PROCEDUREUltrasonic irrigation
Ultrasonic irrigation will be used as a complementary step to chemomechanical procedures to test whether it can improve root canal disinfection.
Sponsors
University of Sao Paulo
Study design
Allocation
NA
Intervention model
SINGLE_GROUP
Primary purpose
TREATMENT
Masking
NONE
Eligibility
Sex/Gender
ALL
Age
18 Years to 65 Years
Healthy volunteers
No
Inclusion criteria
* Teeth with necrotic pulps and asymptomatic apical periodontitis
Exclusion criteria
* patients who had received antibiotics during the previous 3 months or had any general disease, * teeth that could not be properly isolated with rubber dam, * non-restored teeth, * periodontal pockets depths greater than 4 mm, * previous endodontic treatment, * open apex, * crown/root fracture * root resorption or calcifications.
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Bacterial Activity | through study completion, an average of 3 years | The metabolic activity of total bacteria in root canal samples after ultrasonic irrigation |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| Bacterial Levels | through study completion, an average of 3 years | Quantitative data of total bacteria determined by quantitative polymerase chain reaction after endodontic procedures |
Countries
Brazil
Outcome results
None listed