Wart, Genital, Warts Hand
Conditions
Brief summary
In the present study the investigators assessed the invitro role of IL-4 and IFN-γ in predicting the response to bivalent HPV vaccine after whole blood stimulation
Detailed description
40 Patients and 40 controls are enrolled in the study. Participants suffer from chronic recurrent cutaneous and genital warts. Blood samples were withdrawn then whole blood cultures are prepared. Cultures are stimulated with bivalent HPVvaccine. IL-4 and IFN-γ measurement in culture supernatants by ELISA. IL-4 and IFN-γ are investigated to predict the therapeutic response to bivalent HPV vaccine immunotherapy in warts.
Interventions
BIOLOGICALIL-4 and IFN-γ measurements
Blood samples are withdrawn from participants and whole blood cultures are prepared and stimulated by bivalent HPV vaccine.IL-4 and IFN-γ are measured in culture supernatants
Sponsors
Zagazig University
Study design
Observational model
CASE_CONTROL
Time perspective
PROSPECTIVE
Eligibility
Sex/Gender
ALL
Age
36 Months to No maximum
Healthy volunteers
No
Inclusion criteria
* Patients with recurrent and recalcitrant cutaneous and anogenital warts.
Exclusion criteria
* History of allergy to the HPV vaccine * Active viral, fungal, or bacterial infections * Immunosuppressive diseases or drugs. * Autoimmune diseases, or other systemic diseases, e.g., hepatic, or renal disorders, diabetes, meningitis or convulsions * Skin allergies * Pregnancy and lactation * Earlier wart treatment at least one month before enrollment.
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Change of IFN-γ and IL-4 levels | 48 hours | Quantitative measurement of IFN-γ and IL-4 in culture supernatants |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| Change in the size of warts | 6 months | Clinical observation and photographing of wart size |
Countries
Egypt
Outcome results
None listed