Acute Ischemic Stroke
Conditions
Keywords
circRNA
Brief summary
CRAST is to analyze the expression pattern of circular RNA (circRNA) by bioinformatics analysis in patients with acute ischemic stroke and healthy control. The candidate circRNA will be verified as biomarkers for the detection and prognosis of acute ischemic stroke.
Detailed description
Circular RNAs have been highlighted to be involved in the pathological process of ischemic stroke (IS). The purpose of this protocol will investigate the expression pattern of circular RNA (circRNA) by bioinformatics analysis in patients with acute ischemic stroke and healthy control. The candidate circRNA will be verified as biomarkers for the detection and prognosis of acute ischemic stroke. Distinctive expression patterns of circRNA will be identified by individual quantitative real time polymerase chain reaction (qRT-PCR). A diagnostic or predictive model will be established using logistic regression. The panel of these altered circRNAs may be associated with acute IS and could serve as a novel diagnostic or predictive method.
Interventions
GENETICQuantitative Real-time polymerase chain reaction
Quantitative Real-time polymerase chain reaction of circular RNA (circRNA).
Sponsors
The Affiliated Hospital of Xuzhou Medical University
Study design
Observational model
CASE_CONTROL
Time perspective
PROSPECTIVE
Eligibility
Sex/Gender
ALL
Age
18 Years to No maximum
Inclusion criteria
* Aged 18 years or older * Confirmed acute ischemic stroke by a diffusion-weighted imaging-position lesion on magnetic resonance imaging (MRI) and a new lesion on a brain computed tomography (CT) scan * Within 72 hours of symptom onset * Good performance status * Signed an approved informed consents
Exclusion criteria
* a history of hemorrhagic infarction, chronic kidney/liver diseases, peripheral arterial occlusive disease, active malignant disease, and inflammatory or infectious diseases
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Differential expression pattern of circRNA | 90 days | Differential expression pattern of circular RNA (circRNA) will be compared between AIS group and HC group, thus candidate circRNA will be verified as biomarkers for the detection and prognosis of AIS. |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| Dynamic changes of circRNA during the follow-up period | 90 days | Dynamic changes of circRNA will be explored before and after endovascular therapy |
| Correlation of circRNA and stroke-associated infection | 30 days | Correlation of circRNA and stroke-associated infection will be explored in acute ischemic stroke. |
| Correlation of circRNA and inflammatory factors in acute ischemic stroke | 7 days | Correlation of circRNA and inflammatory factors on admission will be explored in acute ischemic stroke. |
| Prognostic value of circRNA in acute ischemic stroke | 90 days | The prognosis of AIS patients will be analyzed between differential expressed candidate circRNA after they have completed the systematic therapy. |
Countries
China
Contacts
Primary ContactXingzhi Wang, MD
Outcome results
None listed