Correlation of cFDNA During CPB to Laboratories SIRS Parameters and Clinical Outcomes
Conditions
Keywords
cfDNA-Circulating cell-free DNA, CPB-cardiopulmonary bypass, SIRS-systemic inflammatory response syndrome
Brief summary
To characterize the changes in circulating cell-free DNA (cfDNA) levels during cardiopulmonary bypass (CPB) and to access their association with outcomes compared to standard scores.
Detailed description
In a prospective study, the investigators enrolled seventy-one consecutive patients undergoing non-emergent coronary artery bypass grafting. Blood was drawn at baseline, after 20 and 40 minutes on CPB, after cross-clamp removal, and 30 minutes after chest closure. cfDNA was measured in sera by fluorescent method. The investigators hypothesize that cfDNA measured by will reflect the magnitude of inflammation induced by CPB. Thus, these measurements could serve for the real-time prognosis of patients undergoing cardiac surgery. The investigators correlate cFDNA to laboratories and clinical outcomes.
Interventions
DIAGNOSTIC_TESTBlood samples
5 blood samples for each patient during CABG (coronary artery bypass grafting) surgery pre/on/post cardiopulmonary bypass machine
Sponsors
Soroka University Medical Center
Study design
Observational model
CASE_CONTROL
Time perspective
PROSPECTIVE
Eligibility
Sex/Gender
ALL
Age
18 Years to No maximum
Healthy volunteers
No
Inclusion criteria
* Elective coronary artery bypass grafting surgery
Exclusion criteria
* Age \< 18 * Non elective surgery * other than CABG surgery * Redo CABG
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| prolong LOS-length of stay | > 18 hours after the surgery | intensive care unit length of stay |
| Mortality | short term - 30day; long term > 1year | Mortality |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| Neutrophil-NETosis | 2 hours after the surgery | Neutrophil extracellular traps (NETs) released from activated neutrophils that undergo NETosis. |
Outcome results
None listed