Test if Increased Chewing Will Increased Microbiota SCFA Production
Conditions
Brief summary
This study is designed to test the effects of chewing efficiency on human gut microbiome composition and function.
Detailed description
Our working hypothesis is that increased chewing will lead to an increase in short-chain fatty acid production We further expect this effect to lead to a detectable decrease in fecal particle size and distinct changes in gut microbial community composition. Here, we will explore this hypothesis using a fixed-order within-subjects study design in a group of healthy participants. Each participant will have one week of normal dietary habits and one week of increased chewing time. We will collect stool samples three times per week in order to measure microbial abundance and metabolism.
Interventions
BEHAVIORALIntervention
Participants will have one week of increased chewing time (chew food until they reach an apple sauce consistency)
Sponsors
Duke University
Study design
Allocation
NA
Intervention model
SINGLE_GROUP
Primary purpose
BASIC_SCIENCE
Masking
NONE
Eligibility
Sex/Gender
ALL
Age
18 Years to No maximum
Healthy volunteers
Yes
Inclusion criteria
* Above age 18? * Able to provide stool samples at no risk to yourself? * Do you have card access to the CIEMAS building (work in CIEMAS, in Pratt School, or undergraduate at Duke), and are you able to visit this building (at times of your own convenience) for the purposes of this study?
Exclusion criteria
* Oral antibiotic treatment within the past month? * Have a history or current diagnosis of irritable bowel syndrome? * Have a history or current diagnosis of inflammatory bowel disease? * Have a history or current diagnosis of type 2 diabetes? * Have a history or current diagnosis of chronic kidney disease with decreased kidney function? * Have a history or current diagnosis of intestinal obstruction? * Have a history or current diagnosis of untreated colorectal cancer? * Have you had a colonoscopy within the past month? * Had oral antibiotics within the past month? * Do you consume one entire non-snack meal or more per week in liquid form (smoothie, meal replacement drink, etc.)? * Do you have any dental issues or other physical limitations that would prevent you from thoroughly chewing your food? * Do you currently practice any mindfulness or dietary techniques that involve making sure that your food is chewed thoroughly?
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| • Change in short-chain fatty acid (SCFA) measured in stool samples by gas chromatography | 1 week baseline and 1 week intervention |
Secondary
| Measure | Time frame |
|---|---|
| Change in median fecal particle size as measured by laser diffraction | 1 week baseline and 1 week intervention |
| Change in gut microbiome composition as measured by 16S rRNA-encoding gene amplicon sequence analysis | 1 week baseline and 1 week intervention |
Countries
United States
Outcome results
None listed