Lupus Nephritis
Conditions
Brief summary
The aim of this study was to evaluate whether C4d is a better biomarker and examine whether C4d plasma levels correlate with treatment response and C4d kidney deposition in systemic lupus erythematosus (SLE) with lupus nephritis (LN).
Detailed description
Evaluation of C4d in lupus nephritis in children C4d level difference in children with systemic lupus with and without renal affection C4d level in lupus nephritis at activity and after remission Detection of C4d deposition in renal tissue relation to disease activity
Interventions
COMBINATION_PRODUCTC4d evaluation in lupus nephritis in serum and renal tissue
C4d plasma levels were analyzed by a unique assay specifically detecting C4d arising from complement activation and C4 plasma levels were quantified with competitive ELISA. SLE patients in activitity with and without lupus nephritis In patient with lupus nephritis we will evaluate the C4d after developing remission in the form of reduction of proteinurea less than .5g/g measured as PCR as complete response and less than50% reduction in proteinurea in partial response according to KIDGO guide line Percutaneous renal biopsies were performed in SLE and non-SLE patients under ultrasonographic guidance. 10% formalin-fixed tissue was embedded in paraffin. Four μm-thick sections were stained with hematoxylin and eosin (H&E), periodic acid-Schiff (PAS), periodic acid methenamine silver (PAM), masson-trichrome and polyclonal rabbit anti-human C4d antibody, Renal biopsy specimens of SLE patients were assessed using the most recent modification of the World Health Organization (WHO)
Sponsors
Assiut University
Study design
Observational model
OTHER
Time perspective
CROSS_SECTIONAL
Eligibility
Sex/Gender
ALL
Age
1 Years to 18 Years
Inclusion criteria
* This will include children and adolescents who fulfill the 2012 Systemic Lupus International Collaborating Clinics (SLICC)(10) classification criteria of SLE. * Inclusion Criteria: * Age younger than 18 years. * Active SLE with and without renal affection * Patients with biopsy proven LN according to ISN/RPS classification criteria of Lupus nephritis.
Exclusion criteria
* -Patients in remission.
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| Evaluation of C4d in lupus nephritis in children | 2 years |
Contacts
Primary ContactAya Khalifa, assisstant lecterurer
Backup ContactAhalam Ali, assisstant professor
Outcome results
None listed