Urea Cycle Disorder, Uremia
Conditions
Brief summary
This project will examine the dysregulation of the urea cycle in patients with terminal uremia using a validated method named "Functional Hepatic Nitrogen Clearance"
Interventions
OTHERAminoacid, alanine-infusion
During 3 hours, patient will receive alanine-infusion, while amino-acid and urea will be monitored by blood samples.
Sponsors
University of Aarhus
Study design
Allocation
NA
Intervention model
SINGLE_GROUP
Primary purpose
TREATMENT
Masking
NONE
Eligibility
Sex/Gender
ALL
Age
18 Years to No maximum
Healthy volunteers
Yes
Inclusion criteria
* Age \> or equal to 18 years * Chronic hemodialysis during 3 months * Patients with functioning arteriovenous-fistula assigned to "Center-Hemodialysis" at "Nyresygdomme, AUH Skejby"
Exclusion criteria
* Active infection * Conditions with vomiting or diarrhea * Diabetes * Active disease being treated with chemotherapy, radiationtherapy, biologic- or similar treatments. Disease under observation can be included. * Liver disease * Vessels on the upper extremities not suited for cannulation * Prednisolone treatment during the last 8 weeks * Pregnancy * BMI \> or equal to 30 or \< or equal to 18 * Physically or mentally condition that does not allow the project to be completed * Language-difficulties that does not allow the project to be completed
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Urea synthesis rate during alanine infusion | 4 hours. 1 hour of blood sample measuring of baseline urea-synthesis rate and 3 hours of urea-synthesis rate during alanine-infusion | Change in the synthesis of urea during a 3 hour period of alanine-infusion in pre and post hemodialysis patients. We will use a validated method named functional hepatic nitrogen clearance (FHNC) to evaluate the synthesis of urea. FHNC is calculated using the linear relation between urea nitrogen synthesis rate and blood-amino acid which is measured every hour. An estimated group of 10 dialysis patients will be included and compared to a clinical healthy group. The healthy group has also received alanine infusion and blood samples of urea and amino-acids has likewise been measured for every hour of infusion. |
Countries
Denmark
Outcome results
None listed