Keratoconus
Conditions
Keywords
Thyroid dysfunction, Vitamin D, Polymorphism, keratoconus
Brief summary
This prospective study the thyroid gland dysfunction and vitamin D receptor polymorphism in keratoconus patients
Detailed description
This is a prospective observational study in which blood samples are collected from keratoconus patients looking for thyroid dysfunction and vitamin D levels in the serum and assessing the Vitamin D receptor polymorphism in comparison to healthy control.
Interventions
DIAGNOSTIC_TESTELISA,DNA extraction
quantitative measurement of (Thyroid stimulating hormon (TSH), free triiodothyronine (FT3)& Free tetraiodothyronine(FT4)) measured using ELISA . Serum 25-OH vitamin D measured using ELISA. Serum 25-OH vitamin D will be classified based on 2012 American Endocrine Society guidelines into deficient (\< 20ng/ml), insufficient ( 21 -30 ng/ml) and sufficient (\> 30 ng/ml).vitamin D receptor polymorphisms was done in 3 steps: a) DNA extraction from whole blood using Gene Jet gene DNA Purification kits . b) PCR: three Vitamin D receptor polymorphisms were tested \[Taq I (restriction enzymes according to single nucleotide polymorphism ) (rs731236), Apa I (rs7975232) and Bsm I (rs1544410)\] using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). Genomic DNA was amplified using certain primers
Sponsors
Mansoura University
Study design
Observational model
CASE_CONTROL
Time perspective
PROSPECTIVE
Eligibility
Sex/Gender
ALL
Age
18 Years to No maximum
Healthy volunteers
Yes
Inclusion criteria
* keratoconus patients without previously diagnosed thyroid disorder in comparison to healthy control without known systemic disease and with normal cornea who were selected from candidates of refractive surgery with normal topography
Exclusion criteria
* patients who could not provide informed consent or the necessary samples for any reason, patients with associated other ophthalmic pathologies or systemic disorders were excluded from the study
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| concentration of free T3 | 2month | pg/ml |
| concentration of free T4 | 2 month | ng/dl |
| concentration of TSH | 2 month | milli-international units per liter |
| vitamin D receptor polymorphism | 2 month | number of participant with vitamin D receptor polymorphism |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| serum vit D | 2 month | concentration of Serum 25 OH vitamin D (ng /ml) |
Countries
Egypt
Outcome results
None listed