Microplastic Levels in Placenta and Cord Blood
Conditions
Brief summary
In this study, microplastic levels in the placenta and cord blood of pregnant women with fetal growth retardation and healthy pregnant women will be compared in placenta and cord blood samples after delivery.
Detailed description
The study will be conducted with a total of 40 pregnant women, 20 of whom are in the control groups and those with FGR diagnoses. Cases in both groups will be matched in terms of demographic information. Demographic information (including socioeconomic status, educational level, region of residence), ultrasound and Doppler examination results, newborn weight at birth, APGAR score, gender, mode of delivery, whether there is a need for neonatal intensive care or not will be recorded.
Interventions
OTHERAnalyzing of microplastic level
Blood samples will be taken from the vein of the umbilical cord. Placental tissues will be taken from the maternal side of the placenta. Placental tissues and serum samples will be stored in the temperature set to -80 °C until analysis. Extraction methods involving chemical reactions will be used to separate and purify microplastics in placenta and cord blood samples. After the extraction process, analysis of microplastic levels in tissues and serum will be done by µ-Raman spectroscopy.
Sponsors
Kutahya Health Sciences University
Study design
Observational model
CASE_CONTROL
Time perspective
PROSPECTIVE
Eligibility
Sex/Gender
FEMALE
Age
18 Years to 40 Years
Healthy volunteers
Yes
Inclusion criteria
* Between the ages of 18-40 years * Gestational age between 320/7 - 396/7 weeks * Singleton pregnancy * For the FGR group: estimated fetal weight \<10th percentile * For the control group: being healthy pregnant * Intact amniotic membrane
Exclusion criteria
\-
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Analyzing of microplastic levels in the placenta and cord blood of pregnant women | 1 year | Microplastic levels in pregnant women's placenta and cord blood will be measured in placenta and cord blood samples after delivery. |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| Investigation of the effect of microplastic level in placenta and cord blood on FGR | 1 month | If there is a significant difference in other data, including the microplastic level, in the FGR group, the effect of each factor on the development of FGR will be analyzed by multi logistic regression analysis. In addition, if the microplastic level is found to be significantly higher in the FGR group, the cut-off value will be obtained by ROC analysis. |
Outcome results
None listed