Metabolic Disturbance
Conditions
Keywords
Isothiocyanates, Blood lactate, Glucose regulation, Nrf2
Brief summary
Many sprouts, for example broccoli sprouts, contain substances that have shown to have protective effects, both against oxidative stress and against carcinogenic substances. In this study we will investigate the activation of the body's antioxidant defense system and effect on blood lactate and glucose after 1 week of isothiocyanate rich sprout supplementation. The subjects will perform maximal and submaximal work test, blood sampling, muscle biopsies and blood pressure monitoring post supplementation. We hypothesize that 1 week of supplementation of isothiocyanate rich sprouts alters blood lactate and glucose metabolism and activates the body's antioxidant defense system.
Detailed description
Many sprouts, for example broccoli sprouts, contain high levels of glucosinolates, which can be converted to the bioactive substances isothiocyanates. This conversion is assisted by another plant enzyme, myrosinase, which is released when the plant is mechanically processed. It is believed that this reaction is part of the plant's natural defense against pest insects and other animals that attack the plant in the early developmental stage. In cell and animal studies supplementation with isothiocyanates has been shown to induce the protein Nrf2 which is a so-called Master regulator of the body's antioxidant defenses and activates more than 200 genes that together provide a strong protection against oxidative and mutagenic stress. In this study we will investigate Nrf2 activation in human skeletal muscle and effect on lactate-, glucose metabolism after 1 week of isothiocyanate rich sprout supplementation. The study is a double-blinded, randomized, placebo controlled, cross-over study with 4 weeks wash-out between the interventions. We will perform maximal- and submaximal work test and monitor blood glucose and lactate during the tests. Also, in the post intervention phases we will perform muscle biopsies, glucose tolerance tests and monitor blood pressure. The hypothesis is that 1 week of isothiocyanate rich sprout supplementation activates Nrf2 in human skeletal muscle and alters blood lactate and glucose metabolism in rest and during exercise.
Interventions
DIETARY_SUPPLEMENTIsothiocyanate rich sprouts
Isothiocyanate rich sprout juice consumption for 1 week
DIETARY_SUPPLEMENTPlacebo Juice
Sprout juice devoid of isothiocyanates for 1 week
Sponsors
The Swedish School of Sport and Health Sciences
Study design
Allocation
RANDOMIZED
Intervention model
CROSSOVER
Primary purpose
PREVENTION
Masking
DOUBLE (Subject, Investigator)
Masking description
The subjects will receive a sprout juice or a placebo juice made of alfalfa sprouts.
Intervention model description
Randomized, placebo controlled, cross-over study
Eligibility
Sex/Gender
ALL
Age
18 Years to 45 Years
Healthy volunteers
Yes
Inclusion criteria
* Healthy volunteer * 18-45 years old
Exclusion criteria
* Any chronic disease * Inability to perform cycling exercise * Allergic to anesthesia
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Blood lactate | Acute during sub maximal exercise | Changes in blood lactate during exercise |
| Nrf2 activation | Acute after 1 week of supplementation | Increased Nrf2 protein in human skeletal muscle |
| Glucose regulation | Throughout 1 week of supplementation | Improved continuous glucose control |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| Glucose tolerance test | 120 minutes | Improved glucose regulation in rest |
| Clinic blood pressure | Acute after 1 week of supplementation | Blood pressure regulation |
Countries
Sweden
Outcome results
None listed