Acute Lung Injury
Conditions
Brief summary
The exosomes in the experimental group and the control group were sequenced to find the difference of the two groups, providing a basis for subsequent basic research.
Detailed description
This study is an observational study, mainly collected by infection, trauma, burns and other non cardiac causes without any related comprehensive character drug treatment of acute respiratory distress (ARDS) in patients with blood, urine or alveolar lavage fluid specimens, used to extract exosomes for omics sequencing, relevant histomorphic variations in exosomes of this disease were obtained, In addition, functional verification of genes or proteins with different changes will be carried out to provide clinical basis for subsequent basic research.
Interventions
DIAGNOSTIC_TESTThe lungs causes and extrapulmonary factors
Acute lung injury due to internal and external pulmonary causes can be used as intervention items in this study, and intervention items can be excluded according to inclusion criteria and exclusion criteria.
Sponsors
Nanfang Hospital, Southern Medical University
Study design
Observational model
CASE_CONTROL
Time perspective
PROSPECTIVE
Eligibility
Sex/Gender
ALL
Age
18 Years to No maximum
Healthy volunteers
Yes
Inclusion criteria
Inclusion criteria of experimental group: * Signed informed consent voluntarily; * Age: \>18 years old, gender: no restrictions ; * Patients with known clinical manifestations of respiratory impairment or new/aggravated respiratory symptoms diagnosed within one week of onset according to the Berlin definition guidelines. Control group inclusion criteria: * Age ≥18 years old (including 18 years old), male and female; * Male weight ≥50 kg, female weight ≥45kg, and body mass index (BMI) between 19 and 26(inclusive), BMI= weight (kg)/ height (㎡); * No smoking, alcohol addiction, no history of drug abuse; * No diabetes, asthma, copd, chronic renal insufficiency and other basic diseases; No history of major diseases or infectious diseases (including hepatitis B, hepatitis C, HIV, syphilis); * Those who have not taken drugs recently.
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Compare the omics differences of blood samples between the experimental and control groups | one year | Collected separately in the experimental group and the control group participants' blood as a specimen, It is used to obtain exosomes and perform omics sequencing, to obtain the histopathological differences of exosomes between experimental and control groups, In addition, functional verification of genes or proteins with different changes will be carried out to provide clinical basis for subsequent basic research . |
| Compare the omics differences of urine samples between the experimental and control groups | one year | Collected separately in the experimental group and the control group participants' urine as a specimen, It is used to obtain exosomes and perform omics sequencing, to obtain the histopathological differences of exosomes between experimental and control groups, In addition, functional verification of genes or proteins with different changes will be carried out to provide clinical basis for subsequent basic research . |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| Compare the omics differences of alveolar lavage fluid samples between the experimental and control groups | one year | Collected separately in the experimental group and the control group participants' alveolar lavage fluid as a specimen, It is used to obtain exosomes and perform omics sequencing, to obtain the histopathological differences of exosomes between experimental and control groups, In addition, functional verification of genes or proteins with different changes will be carried out to provide clinical basis for subsequent basic research . |
Countries
China
Contacts
Primary Contactzhuping M zheng, bachelor
Outcome results
None listed